RHYTHM: An Open Source Imaging Toolkit for Cardiac Panoramic Optical Mapping
RHYTHM: An Open Source Imaging Toolkit for Cardiac Panoramic Optical Mapping
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DOI：
10.1038/s41598-018-21333-w
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发表时间：
2018-02-13
影响因子：
4.6
通讯作者：
Efimov, Igor R.
中科院分区：
文献类型：
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作者：
Gloschat, Christopher;Aras, Kedar;Efimov, Igor R.
荧光光学成像技术已经彻底改变了心脏电生理学领域，并推进了我们对心律失常等复杂电活动的理解。然而，传统的单目光学映射系统尽管具有高空间分辨率，但受限于二维（2D）视场。因此，跟踪复杂的三维（3D）电波（诸如在心室纤颤期间）是具有挑战性的，因为波快速地移入和移出视场。这个问题已经通过全景成像解决，全景成像使用多个相机来测量整个心外膜表面的电活动。然而，设计和实施该解决方案所需的各种工程技能和大量资源成本使其在很大程度上无法进入整个生物医学研究界。为了解决这个进入障碍，我们提出了一个用于构建全景光学映射系统的开源工具包，其中包括灌注和成像硬件的3D打印，以及用于数据处理和分析的软件。在本文中，我们描述了工具包，并证明它在不同的哺乳动物心脏：小鼠，大鼠和兔。
Fluorescence optical imaging techniques have revolutionized the field of cardiac electrophysiology and advanced our understanding of complex electrical activities such as arrhythmias. However, traditional monocular optical mapping systems, despite having high spatial resolution, are restricted to a twodimensional (2D) field of view. Consequently, tracking complex three-dimensional (3D) electrical waves such as during ventricular fibrillation is challenging as the waves rapidly move in and out of the field of view. This problem has been solved by panoramic imaging which uses multiple cameras to measure the electrical activity from the entire epicardial surface. However, the diverse engineering skill set and substantial resource cost required to design and implement this solution have made it largely inaccessible to the biomedical research community at large. To address this barrier to entry, we present an open source toolkit for building panoramic optical mapping systems which includes the 3D printing of perfusion and imaging hardware, as well as software for data processing and analysis. In this paper, we describe the toolkit and demonstrate it on different mammalian hearts: mouse, rat, and rabbit.