Pulse-length dependence of cellular response to intense near-infrared laser pulses in multiphoton microscopes
Pulse-length dependence of cellular response to intense near-infrared laser pulses in multiphoton microscopes
复制标题
DOI：
10.1364/ol.24.000113
复制
发表时间：
1999-01-15
期刊：
影响因子：
3.6
通讯作者：
Halbhuber, KJ
中科院分区：
文献类型：
--
作者：
König, K;Becker, TW;Halbhuber, KJ
研究了780和920 nm超短激光脉冲的脉冲长度τ对细胞活力和细胞繁殖的影响。总共2400个未标记的细胞暴露于来自锁模80 MHz钛：蓝宝石激光器的高度聚焦的扫描光束，像素停留时间为60 μ s。对于相同的脉冲能量，破坏性的影响更明显的较短的脉冲。发现损伤行为大致遵循P-2/tau依赖性（P，平均功率），表明细胞破坏可能基于双光子激发过程而不是单光子或三光子事件。因此，飞秒以及皮秒脉冲提供了大致相同的相对光学窗口安全的双光子荧光显微镜。(C)1999年美国光学学会。
The influence of the pulse length, tau, of ultrashort laser pulses at 780 and 920 nm on cell vitality and cellular reproduction has been studied. A total of 2400 nonlabeled cells were exposed to a highly focused scanning beam from a mode-locked 80-MHz Ti:sapphire laser with 60-mu s pixel dwell time. For the same pulse energy, destructive effects were more pronounced for shorter pulses. The damage behavior was found to follow approximately a P-2/tau dependence (P, mean power), indicating that cell destruction is likely based on a two-photon excitation process rather than a one- or a three-photon event. Therefore, femtosecond as well as picosecond pulses provide approximately the same relative optical window for safe two-photon fluorescence microscopy. (C) 1999 Optical Society of America.