Detection of antibodies against the Mycoplasma bovis glyceraldehyde-3-phosphate dehydrogenase protein in beef cattle
Detection of antibodies against the Mycoplasma bovis glyceraldehyde-3-phosphate dehydrogenase protein in beef cattle
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DOI：
10.1016/j.micpath.2007.05.009
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发表时间：
2007-11-01
影响因子：
3.8
通讯作者：
Prysliak, Tracy
中科院分区：
文献类型：
--
作者：
Perez-Casal, Jose;Prysliak, Tracy
牛支原体引起的疾病是肉牛和奶牛生产者经济损失的重要来源。M.牛痘病毒阻碍了有效疫苗的生产，尽管可用的疫苗很少，但它们是由从少数分离株获得的细菌制备的，这可能限制了它们的有效性。因此，开发一种疫苗，保护所有的M。牛分离物时，必须使用显示较少或没有抗原变异的共同抗原。我们分离了M.牛编码甘油醛-3-磷酸脱氢酶（GAPDH），并表明牛与M。牛能够对GAPDH产生免疫应答。利用限制性片段长度多态性（RFLP）技术，对几种M.通过PCR扩增的牛缺口基因，我们能够检测到小的基因内变异，使我们能够将基因分为两组，而不改变蛋白质的抗原组成。结合牛的免疫反应和蛋白质的抗原保守性，表明M。牛GAPDH蛋白可能是一个潜在的靶点，用于开发更有效的抗所有M.牛分离物。(C)2007爱思唯尔有限公司保留所有权利。
Diseases caused by Mycoplasma bovis are an important source of financial losses for beef and dairy cattle producers. Antigenic variation in M. bovis hinders the production of effective vaccines and although there are few vaccines available, they are prepared from bacteria obtained from few isolates potentially limiting their effectiveness. Thus, to develop a vaccine that protects against all M. bovis isolates, it is necessary to use a common antigen that shows less or no antigenic variation. We have isolated the gap gene of M. bovis encoding for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and showed that cattle colonized with M. bovis were able to mount an immune response to GAPDH. Using restriction-fragment length polymorphism (RFLP) of several M. bovis gap genes amplified by PCR, we were able to detect small intragenic variations that allowed us to classify the genes into two groups without changing the antigenic makeup of the proteins. The immune responses detected in cattle combined with the antigenic conservation of the proteins suggest that the M. bovis GAPDH protein could be a potential target for development of a more effective vaccine against all M. bovis isolates. (C) 2007 Elsevier Ltd. All rights reserved.