Live cell analysis at sea reveals divergent thermal performance between photosynthetic ocean microbial eukaryote populations
Live cell analysis at sea reveals divergent thermal performance between photosynthetic ocean microbial eukaryote populations
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DOI：
10.1038/s41396-019-0355-6
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发表时间：
2019-01
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通讯作者：
A. McInnes;O. Laczka;Kirralee G Baker;Michaela E. Larsson;C. Robinson;Jennifer S. Clark;L. Laiolo;Marco Alvarez;B. Laverock;Colin T. Kremer;E. van Sebille;M. Doblin
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作者：
A. McInnes;O. Laczka;Kirralee G Baker;Michaela E. Larsson;C. Robinson;Jennifer S. Clark;L. Laiolo;Marco Alvarez;B. Laverock;Colin T. Kremer;E. van Sebille;M. Doblin
海上实验可以深入了解海洋微生物的哪些特征与原位性能相关。在这里，我们使用活性氧（ROS）荧光标记确定了在西南太平洋采样的邻近水团中微生物光养生物的耐热性的独特模式。孵育 1、5 和 25 小时后，沿着温度梯度（15.6–32.1°C）评估微微真核生物的 ROS 含量。来自东澳大利亚海流 (EAC) 的微微真核生物具有相对恒定的 ROS，在低于环境温度 7°C 的情况下 25 小时后死亡率最高，而来自塔斯曼海的微微真核生物在极端温暖和寒冷的温度下都具有升高的 ROS，并且在高于环境温度 6-10°C 时死亡率最高，这被解释为热应激的结果。海洋环流模型中水团的跟踪显示，种群具有不同的热历史，EAC 超微型真核生物在采样前至少 1 周经历了较高的平均温度。虽然适应和群落组装都可能影响生物反应，但这项研究清楚地表明，表型分歧沿着浮游漂流轨迹发生。
Experimentation at sea provides insight into which traits of ocean microbes are linked to performance in situ. Here we show distinct patterns in thermal tolerance of microbial phototrophs from adjacent water masses sampled in the south-west Pacific Ocean, determined using a fluorescent marker for reactive oxygen species (ROS). ROS content of pico-eukaryotes was assessed after 1, 5 and 25 h of incubation along a temperature gradient (15.6–32.1 °C). Pico-eukaryotes from the East Australian Current (EAC) had relatively constant ROS and showed greatest mortality after 25 h at 7 °C below ambient, whereas those from the Tasman Sea had elevated ROS in both warm and cool temperature extremes and greatest mortality at temperatures 6–10 °C above ambient, interpreted as the outcome of thermal stress. Tracking of water masses within an oceanographic circulation model showed populations had distinct thermal histories, with EAC pico-eukaryotes experiencing higher average temperatures for at least 1 week prior to sampling. While acclimatization and community assembly could both influence biological responses, this study clearly demonstrates that phenotypic divergence occurs along planktonic drift trajectories.