Human and mouse gene structure: comparative analysis and application to exon prediction
Human and mouse gene structure: comparative analysis and application to exon prediction
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DOI：
10.1145/332306.332326
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发表时间：
2000-04
期刊：
影响因子：
7
通讯作者：
S. Batzoglou;L. Pachter;J. Mesirov;B. Berger;E. Lander
中科院分区：
文献类型：
--
作者：
S. Batzoglou;L. Pachter;J. Mesirov;B. Berger;E. Lander
我们描述了一种新的基于跨物种比较的基因识别分析方法。我们首先比较了人类和小鼠的正向同源基因组外观，研究了外显子和内含子的数量、大小和序列的相似程度。然后我们开发了一种识别这种正向同源区域内基因的方法，通过首先使用迭代全局比对系统比对区域，然后基于两个物种中比对位置处外显子特征的保守性来鉴定基因。分别称为GLASS和ROSETTA的程序ROSETTA在测试的117个正向同源序列对中的编码外显子的精确鉴定方面表现良好。
We describe a novel analytical approach to gene recognition based on cross-species comparison We first undertook a comparison of orthologous genomic look from human and mouse, studying the extent of similarity in the number, size and sequence of exons and introns We then developed an approach for recognizing genes within such orthologous regions, by first aligning the regions using an iterative global alignment system and then identifying genes based on conservation of exonic features at aligned positions in both species The alignment and gene recognition are performed by new programs called GLASS and ROSETTA, respectively ROSETTA performed well at exact identification of coding exons in 117 orthologous pairs tested.