ACTH-induced hypertension in rats: fact or artifact?
ACTH-induced hypertension in rats: fact or artifact?
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ACTH 诱发的大鼠高血压：事实还是人为因素？
DOI：
10.1152/ajpregu.1989.256.6.r1308
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Callahan,MF
中科院分区：
文献类型：
--
作者：
Gruber,KA;Eskridge-Sloop,SL;Eldridge,JC;Callahan,MF
来自许多实验室的证据表明，给大鼠注射促肾上腺皮质激素(ACTH)会在5天内产生高血压。然而，在这些研究中，血压的分析是通过尾袖技术进行的，这是一种急性和间接的方法。我们现在已经通过皮下注射ACTH(每天5或10U，连续9天)给长期固定在代谢笼中的大鼠注射ACTH。虽然尾袖测量动脉压表明ACTH治疗产生了高血压，但这并不能通过24小时的平均动脉压直接测量来证实。与生理盐水对照组相比，ACTH在整个治疗期间对24小时心率没有影响。我们还检查了所有测量的变异系数。ACTH给药后上述因素均未发生改变。然而，ACTH治疗确实产生了利尿作用，进一步证实了先前的工作，并为ACTH储存库的生物利用度提供了肾脏和心血管方面的证据。这些结果表明，慢性ACTH治疗并不会在大鼠中产生真正的高血压状态，而是可能会增强间接动脉压测量技术的应激反应。
Evidence from numerous laboratories has shown that administration of adrenocorticotropic hormone (ACTH) to rats produces hypertension within 5 days. However, the analysis of blood pressure in these studies was by the tail-cuff technique, an acute and indirect approach. We have now administered ACTH, via a subcutaneous depot injection (5 or 10 U/day for 9 days), to chronically instrumented rats maintained in metabolic cages. Although tail-cuff measurements of arterial pressure indicated that the ACTH treatment produced hypertension, this was not confirmed by direct 24-h measurements of mean arterial pressure. There was no effect of ACTH on 24-h heart rate throughout the treatment period compared with saline-injected controls. We also examined coefficient of variation of all our measurements. None of the factors was altered by ACTH administration. However, ACTH treatment did produce a diuretic effect, further confirming previous work and providing renal, in addition to cardiovascular, evidence for the bioavailability of the ACTH depot. These results demonstrate that chronic ACTH treatment does not produce a true hypertensive state in rats but rather may enhance the cardiovascular response to the stress of the indirect arterial pressure measurement technique.