Some recent advances relating to prokaryotic cytochrome c reductases and cytochrome c oxidases.
Some recent advances relating to prokaryotic cytochrome c reductases and cytochrome c oxidases.
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有关原核细胞色素 c 还原酶和细胞色素 c 氧化酶的一些最新进展。
DOI：
10.1016/s0005-2728(05)80260-9
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Gennis,RB
中科院分区：
文献类型：
--
作者：
Gennis,RB
原核系统为探索复杂的膜结合多亚基酶的结构/功能关系提供了极好的实验机会，这些酶负责呼吸或光合电子传递链中c型细胞色素的还原和随后的氧化。这篇小综述提出了两点：(1)真核和原核a3型细胞色素氧化酶是结构相关的呼吸氧化酶的一个明显大的超家族的成员。该超家族在血红素辅基（aorb）以及氧化底物（醌或细胞色素）方面表现出相当大的变化。这些酶之间的关系有助于促进对它们如何工作的探索。 (2)分子生物学技术可用于生成完整的、氧化还原活性的、水溶性的膜结合亚基结构域。这些可溶域可用于详细检查，包括通过 NMR 技术或 X 射线晶体学获得高分辨率结构。该方法用于研究球形红杆菌bc1复合体中细胞色素c1的可溶性血红素结合域。
Prokaryotic systems provide excellent experimental opportunities for exploring structure/function relationships for the complex, membrane-bound, multisubunit enzymes responsible for the reduction and subsequent oxidation ofc-type cytochromes in respiratory or photosynthetic electron transport chains. Two points are made in this mini-review: (1) The eukaryotic and prokaryoticaa3-type cytochromecoxidases are members of an apparently large superfamily of structurally related respiratory oxidases. This superfamily displays considerable variation in terms of the heme prosthetic groups (aorb) as well as the substrate oxidized (quinol or cytochromec). The relationships among these enzymes help to facilitate explorations of how they work. (2) Molecular biology techniques can be used to generate intact, redox-active, water-soluble domains of membrane-bound subunits. These soluble domains can be used for detailed examination, including obtaining high resolution structure by NMR techniques or by X-ray crystallography. This approach is being used to study the soluble heme-binding domain of cytochromec1from thebc1complex ofRhodobacter sphaeroides.