Circulating T cell repertoire complexity in normal individuals and bone marrow recipients analyzed by CDR3 size spectratyping. Correlation with immune status.
Circulating T cell repertoire complexity in normal individuals and bone marrow recipients analyzed by CDR3 size spectratyping. Correlation with immune status.
复制标题
DOI：
10.4049/jimmunol.154.3.1521.a
复制
发表时间：
1994-05
影响因子：
4.4
通讯作者：
Jack Gorski;M. Yassai;X. Zhu;B. Kissela;B. K. B. C. T. Kissela-B.-K.-B.-C.-T.-Kissela-2230401082;C. Keever;N. Flomenberg
中科院分区：
文献类型：
--
作者：
Jack Gorski;M. Yassai;X. Zhu;B. Kissela;B. K. B. C. T. Kissela-B.-K.-B.-C.-T.-Kissela-2230401082;C. Keever;N. Flomenberg
参与局部或全身免疫应答的T细胞库的分析开始在许多临床情况中发挥重要作用。这些包括自身免疫、对病毒或细菌超抗原的应答、同种异体免疫（包括同种异体移植排斥）和肿瘤免疫。在这里，我们通过使用V β家族特异性PCR的修改来确定TCR β链基因的复杂性来分析循环T细胞库。这种方法被称为CDR 3大小谱分析，它使用CDR 3的大小异质性作为TCR分析中特异性的另一个来源。它已被用于分析正常成人（包括骨髓供体和骨髓移植受体）循环T细胞库的复杂性和稳定性。正常的光谱是复杂的和稳定的。骨髓受体的库复杂性与其免疫功能状态相关。在患有与T细胞损伤相关的复发性感染的个体中揭示了剧目的收缩和间隙。谱型分析适用于其他特定库偏斜的研究，例如可能与免疫缺陷相关或在免疫活性部位发现。
The analysis of the T cell repertoires involved in local or systemic immune responses is beginning to play an important role in many clinical situations. These include autoimmunity, response to viral or bacterial superantigens, alloimmunity including allograft rejection, and tumor immunity. Here we analyze circulating T cell repertoires by determining TCR beta-chain gene complexity using a modification of V beta family-specific PCR. This approach, called CDR3 size spectratyping, uses the size heterogeneity of the CDR3 as a further source of specificity in TCR analysis. It has been used here to analyze the complexity and stability of circulating T cell repertoires in normal adults, including bone marrow donors, and bone marrow transplant recipients. Normal spectratypes are both complex and stable. The repertoire complexity of marrow recipients correlates with their state of immune function. Contractions and gaps in repertoires are revealed in individuals suffering from recurrent infections associated with T cell impairment. Spectratype analysis is applicable to other studies of specific repertoire skewing such as may be associated with immunodeficiency or found at sites of immune activity.