Comparison of separation performances of novel β-cyclodextrin-based chiral stationary phases in high-performance liquid chromatographic enantioseparation.
Comparison of separation performances of novel β-cyclodextrin-based chiral stationary phases in high-performance liquid chromatographic enantioseparation.
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DOI：
10.1016/j.jpba.2012.05.023
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发表时间：
2012-11
影响因子：
3.4
通讯作者：
G. Varga;G. Fodor;I. Ilisz;J. Szeman;J. Visy;L. Szente;A. Péter
中科院分区：
文献类型：
--
作者：
G. Varga;G. Fodor;I. Ilisz;J. Szeman;J. Visy;L. Szente;A. Péter
应用新型键合化学开发了三种基于 β-环糊精的手性固定相。比较了 β-环糊精、(R,S)-2-羟丙基-β-环糊精和基于全甲基-β-环糊精的 CSP 在解析结构不同的分析物（如香豆素、丹酰基氨基酸和丙酸衍生物）方面的分离性能。使用不同组成的 0.1% 磷酸三乙铵 (pH 3.5)/MeOH 流动相系统以反相模式进行分离。在三种新型 CSP 中，全甲基-β-环糊精键合相被证明是对研究的分析物进行对映体分离最有效的一种。
Three β-cyclodextrin-based chiral stationary phases were developed applying novel bonding chemistry. The separation performances of β-cyclodextrin, (R,S)-2-hydroxypropyl-β-cyclodextrin, and permethyl-β-cyclodextrin-based CSPs were compared in the resolution of structurally divergent analytes, such as coumarins, dansyl amino acids, and propionic acid derivatives. Separations were carried out in reversed phase mode applying 0.1% triethylammonium phosphate (pH 3.5)/MeOH mobile phase systems in different compositions. Of the three novel CSPs the permethyl-β-cyclodextrin bonded phase proved to be the most effective one for the enantioseparation of investigated analytes.