Modification of polyurethaneurea with PEG and YIGSR peptide to enhance endothelialization without platelet adhesion
Modification of polyurethaneurea with PEG and YIGSR peptide to enhance endothelialization without platelet adhesion
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DOI：
10.1002/jbm.b.30135
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发表时间：
2005-01-15
影响因子：
3.4
通讯作者：
West, JL
中科院分区：
文献类型：
--
作者：
Jun, HW;West, JL
无血小板粘附的内皮化改善对于增强合成血管移植物和其他血液接触装置的长期通畅性至关重要。我们已经开发了一种双重修饰的聚脲，通过将内皮细胞粘附YIGSR肽序列作为扩链剂和非血栓形成PEG作为聚合物主链中的软段（PUUYIGSR-PEG）。成功合成了PUUYIGSR-PEG，并通过质子NMR、FTIR、GPC、DSC、ESCA和接触角测量对其进行了表征。尽管具有相似的分子量，但肽/PEG修饰的聚脲（PUUYIGSR-PEG）与对照PEG修饰的聚脲（PUUPPD-PEG）相比显示出上级机械性能。在PUUYIGSR-PEG上几乎没有观察到血小板粘附，而与PUUPPD-PEG相比，在PUUYIGSR-PEG上的内皮细胞粘附、扩散和迁移显著更大。因此，这种生物活性聚合物可能是小直径血管移植物的合适生物材料。(C)2004 Wiley Periodicals，Inc.
Improved endothelialization without platelet adhesion is essential to enhance the long-term patency of synthetic vascular grafts and other blood-contacting devices. We have developed a dually modified polyurethaneurea by incorporating endothelial cell adhesive YIGSR peptide sequences as chain extenders and nonthrombogenic PEG as a soft segment (PUUYIGSR-PEG) in the polymer backbone. PUUYIGSR-PEG was successfully synthesized and characterized by proton NMR, FTIR, GPC, DSC, ESCA, and contact angle measurement. Despite having similar molecular weight, the peptide/PEG-modified polyurethaneurea (PUUYIGSR-PEG) showed superior mechanical properties compared to the control PEG-modified polyurethaneurea (PUUPPD-PEG). Virtually no platelet adhesion was observed on PUUYIGSR-PEG, while endothelial cell adhesion, spreading, and migration were significantly greater on PUUYIGSR-PEG compared to PUUPPD-PEG. Thus, this bioactive polymer may be an appropriate biomaterial for small diameter vascular grafts. (C) 2004 Wiley Periodicals, Inc.