Existence of Pink1 Antisense RNAs in Mouse and Their Localization
Existence of Pink1 Antisense RNAs in Mouse and Their Localization
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DOI：
10.1159/000251963
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发表时间：
2009-01-01
影响因子：
1.7
通讯作者：
Yasue, H.
中科院分区：
文献类型：
--
作者：
Chiba, M.;Kiyosawa, H.;Yasue, H.
PTEN诱导的激酶1（PINK 1）是由抑癌基因PTEN反式激活的基因，是帕金森病（PD）的致病基因之一。为了理解PD，已经利用了含有受影响的Pink 1（例如功能丧失突变）的啮齿动物模型。最近，PINK 1的天然反义RNA已被证明参与PINK 1基因座的调节。然而，迄今为止，除了人类外，还没有Pink 1的反义RNA的报道。因此，在本研究中，当在小鼠中寻找Pink 1反义RNA时，我们发现反义RNA是从与产生反义RNA的人类区域相对应的小鼠基因组区域转录的。利用原位杂交技术研究了反义RNA在小鼠脑内的定位;这表明反义RNA定位于发现Pink 1 mRNA的脑区域。此外，在新生和1周龄小鼠的海马中发现mRNA和反义RNA比其他脑区域更密集，而这些RNA在胚胎14天、17天和8周龄的小鼠大脑区域中被均匀地发现。版权所有（C）2009 S. Karger AG，巴塞尔
PTEN-induced kinase 1 (PINK1), which is identified as the gene transactivated by the tumor suppressor PTEN, has been found to be one of the causative genes in Parkinson's disease (PD). In order to understand PD, rodent models containing affected Pink1 such as loss-of-function mutations have been exploited. Recently, natural antisense RNA of PINK1 has been demonstrated to be involved in the regulation of the PINK1 locus. However, no antisense RNAs of Pink1 except for human have been reported so far. Therefore, in the present study, while searching for the Pink1 antisense RNAs in mouse, we found that the antisense RNAs are transcribed from a mouse genomic region corresponding to the human region from which the antisense RNAs are produced. Further, we investigated the localization of the antisense RNAs in mouse brain using in situ hybridization; this demonstrated that the antisense RNAs were localized in the regions of brain where the Pink1 mRNA was found. In addition, the mRNA and antisense RNAs were found more densely in the hippocampus than in the other brain regions in newborn and 1-week-old mice, while those RNAs were found uniformly in the mouse brain regions of embryo day (E) 14, E17, and 8-weeks-old. Copyright (C) 2009 S. Karger AG, Basel