Translating daily COVID-19 screening into a simple glucose test: a proof of concept study.
Translating daily COVID-19 screening into a simple glucose test: a proof of concept study.
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将每日COVID-19筛查转化为简单的血糖测试：概念验证研究。
DOI：
10.1039/d1sc00512j
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发表时间：
2021-07-07
期刊：
影响因子：
8.4
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Liu R;Hu Y;He Y;Lan T;Zhang J
家庭检测是一种有吸引力的新兴战略，可以通过在家隔离、筛查和监测症状来对抗COVID-19大流行，并防止医疗资源过载。然而，由于敏感性、可用性和成本之间的权衡，目前的SARS-CoV-2诊断技术仍然存在一些缺陷，使得大多数家庭用户无法负担这项测试。为了解决这些限制，利用聚集规律间隔短回文重复序列（crispr）和便携式血糖仪（PGM），我们提出了一种靶向应答的CRISPR-PGM系统的概念验证演示，该系统可将SARS-CoV-2检测转化为葡萄糖测试。利用该系统，用PGM定量检测了SARS-CoV-2的特异性N基因、N蛋白和伪病毒。鉴于多种生物受体易于整合到CRISPR-PGM系统中，该方法为患者提供了一个起点，可以在家中定量监测多种COVID-19生物标志物的单设备解决方案。COVID-19葡萄糖检测：将目标响应滚动圈扩增和基于crispr的侧支切割模块与便携式血糖仪结合，实现了将SARS-CoV-2检测转化为葡萄糖检测。
Home testing is an attractive emerging strategy to combat the COVID-19 pandemic and prevent overloading of healthcare resources through at-home isolation, screening and monitoring of symptoms. However, current diagnostic technologies of SARS-CoV-2 still suffer from some drawbacks because of the tradeoffs between sensitivity, usability and costs, making the test unaffordable to most users at home. To address these limitations, taking advantage of clustered regularly interspaced short palindromic repeats (CRISPRs) and a portable glucose meter (PGM), we present a proof-of-concept demonstration of a target-responsive CRISPR-PGM system for translating SARS-CoV-2 detection into a glucose test. Using this system, a specific N gene, N protein, and pseudo-viruses of SARS-CoV-2 have been detected quantitatively with a PGM. Given the facile integration of various bioreceptors into the CRISPR-PGM system, the proposed method provides a starting point to provide patients with a single-device solution that can quantitatively monitor multiple COVID-19 biomarkers at home. COVID-19 glucose test: translating SARS-CoV-2 detection into a glucose test is achieved by incorporating target-responsive rolling circle amplification and a CRISPR-based collateral cleavage module with a portable glucose meter.
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