An immunoproteomic approach for characterization of dormancy within Staphylococcus epidermidis biofilms
An immunoproteomic approach for characterization of dormancy within Staphylococcus epidermidis biofilms
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DOI：
10.1016/j.molimm.2015.02.024
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发表时间：
2015-06-01
影响因子：
3.6
通讯作者：
Vitorino, Rui
中科院分区：
文献类型：
--
作者：
Carvalhais, Virginia;Cerveira, Frederico;Vitorino, Rui
表皮葡萄球菌的毒力主要来源于其在留置医疗器械表面的定植和生物膜的形成。生物膜的生理异质性可能影响宿主的免疫反应和对抗生素的敏感性。除其他外，多角体细胞有助于生物膜异质性。本研究的目的是鉴定S.利用双向电泳（2-DE）免疫印迹和质谱（MS）技术，对与休眠机制相关的表皮细胞生物膜进行了初步研究。共鉴定了19种可被人血清样品识别的细菌蛋白。这些蛋白质主要参与小分子代谢生物学过程。催化活性和离子结合是最具代表性的分子功能。CodY和GpmA蛋白更反应血清时，生物膜休眠诱导，而FtnA和ClpP更反应时，休眠被阻止。这是第一个确定细菌生物膜内免疫反应性蛋白质的差异与诱导或阻止休眠的工作。考虑到休眠在生物膜中的重要性，对这些蛋白质的进一步评估可以提供与休眠相关的机制的见解，并有助于提高目前对休眠如何影响宿主免疫反应的理解。(C)2015爱思唯尔有限公司版权所有。
Virulence of Staphylococcus epidertnidis is mainly attributed to surface colonization and biofilm formation in indwelling medical devices. Physiological heterogeneity of biofilms may influence host immune response and sensitivity to antibiotics. Dormant cells, among others, contribute to biofilm heterogeneity. The aim of this study was to identify immunogenic proteins of S. epidermidis biofilms associated with dormancy mechanism, by using two-dimensional electrophoresis (2-DE) immunoblotting and mass spectrometry (MS). A total of 19 bacterial proteins, recognized by human serum samples, were identified. These proteins were mainly involved in small molecule metabolic biological processes. Catalytic activity and ion binding were the most representative molecular functions. CodY and GpmA proteins were more reactive to sera when biofilm dormancy was induced, while FtnA and ClpP were more reactive when dormancy was prevented. This is the first work that identifies differences in immunoreactive proteins within bacterial biofilms with induced or prevented dormancy. Considering the importance of dormancy within biofilms, further evaluation of these proteins can provide insights into the mechanisms related to dormancy and help to improve current understanding on how dormancy affects the host immune response. (C) 2015 Elsevier Ltd. All rights reserved.