Collective Properties Extend Resistance to Photobleaching of Highly Doped PluS NPs
Collective Properties Extend Resistance to Photobleaching of Highly Doped PluS NPs
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DOI：
10.1002/ejic.201700831
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发表时间：
2017-12-01
影响因子：
2.3
通讯作者：
Prodi, Luca
中科院分区：
文献类型：
--
作者：
Genovese, Damiano;Rampazzo, Enrico;Prodi, Luca
染料掺杂的纳米颗粒（NPs）是一种有趣的荧光体系，其中可以产生集体性质，这归因于染料的整体而不是单个染料。可以定制集体性质以增加亮度并引入物理多功能性。在这种背景下，自猝灭一直被认为是要避免的现象。在这里，我们报告了从自猝灭特性中获益的可能性：与掺杂度较低的纳米颗粒相比，每个NP具有大量染料（包括自猝灭染料）的纳米颗粒显示出慢得多的光漂白。通过这种方式，它们的发射强度可以在十倍的时间内保持几乎恒定。这种延长的发光持续时间是由于自猝灭荧光团的优先光漂白。这些观察结果可以在强激发条件下使用高度染料掺杂的纳米颗粒作为持久的，超光稳定的探针。
Dye-doped nanoparticles (NPs) are intriguing fluorescent systems in which collective properties can arise, which are ascribable to the ensemble of dyes rather than to individual ones. Collective properties can be tailored to increase brightness and introduce photophysical versatility. In this context, self-quenching has long been regarded as the phenomenon to avoid. Here we report on the possibility to profit from a property stemming from self-quenching: nanoparticles with a high number of dyes per NP (including self-quenched dyes) display much slower photobleaching compared to nanoparticles with a lower doping degree. In this way, their emission intensity can be kept almost constant for ten times longer. This extended duration of luminescence is due to preferential photobleaching of self-quenched fluorophores. These observations can shine new light on the use of highly dye-doped nanoparticles as long-lasting, super-photostable probes under strong excitation conditions.