Electrochromatographic behavior of core-shell particles: A comparison study
Electrochromatographic behavior of core-shell particles: A comparison study
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核壳颗粒的电色谱行为：比较研究
DOI：
10.1016/j.aca.2018.06.007
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发表时间：
2018
影响因子：
6.2
通讯作者：
Zhang Bo
中科院分区：
文献类型：
--
作者：
Yang Lijun;Xu Lingjia;Guo Rui;Gao Tianyu;Guo Haoxin;Yu Yue;Lv Jijian;Wang Qiuquan;Zhang Bo
核壳粒子是新一代高效液相色谱填料。通过与相同粒径的经典全多孔硅相比较，研究了Ascentis Express 5 μm核壳粒子的电色谱行为，包括电压-电流特性、电渗流和货车-Deemter曲线。结果发现，由于非渗透性固体核，核-壳颗粒呈现出比完全多孔颗粒降低的渗透率和效率。这也从另一方面证明了全多孔材料上颗粒内孔流的广泛存在和在电色谱中的重要作用。核-壳颗粒的高保留性导致弱保留的亲水性肽的分辨率增强，所述弱保留的亲水性肽在完全多孔的颗粒上保留和共洗脱较差。进一步的探索表明，核壳材料可以实现蛋白质的高效电色谱，在分辨率，重现性和长期稳定性方面具有优异的性能。结果表明，这种核壳结构可以作为多肽和蛋白质生物电色谱固定相的合理设计。
Core-shell particle is a new generation high performance packing material for liquid chromatography. Through comparison with a classical totally porous silica phase of the same particle size, we studied Ascentis Express 5 μm core-shell particle's electrochromatographic behavior, in terms of voltage-current property, electroosmotic flow (EOF) and van Deemter curve. It was found, due to the nonpermeable solid core, the core-shell particle presented a diminished EOF and efficiency than the totally porous paricle. This on the other hand proved that the intra-particle pore flow extensively exists and plays an important role in electrochromatography on totally porous material. The core-shell particle's high retentivity led to an enhanced resolution for weakly retained hydrophilic peptides, which were poorly retained and co-eluted on totally porous particles. Further exploration has shown the core-shell material can achieve efficient electrochromatography of protein digests, excellent performance in terms of resolution, reproducibility and long term stability have been observed. The results indicate that the core-shell structure may suggest a reasonable design of stationary phase for bioelectrochromatography of peptides and proteins.