Independent modulation of Arabidopsis thaliana polyubiquitin mRNAs in different organs and in response to environmental changes.
Independent modulation of Arabidopsis thaliana polyubiquitin mRNAs in different organs and in response to environmental changes.
复制标题
DOI：
10.1046/j.1365-313x.1997.11051017.x
复制
发表时间：
1997-05
期刊：
影响因子：
--
通讯作者：
C. Sun;Judy Callis
中科院分区：
文献类型：
--
作者：
C. Sun;Judy Callis
泛素是拟南芥（Arabidopsis thaliana）哥伦比亚生态型中的5个多聚泛素基因编码的一种高度保守的蛋白质，它们被分为两个亚型：UBQ 3/UBQ 4亚型和UBQ 10/UBQ 11/UBQ 14亚型。使用基因特异性寡核苷酸作为杂交探针的北方分析和来自在单个多聚泛素5'侧翼区控制下表达β-葡萄糖醛酸酶（GUS）的转基因植物的酶活性测量用于确定多聚泛素转录和mRNA积累的发育和环境调节。亚型内和亚型之间的聚遍在蛋白mRNA水平受到独立调节。在营养器官中，UBQ 3 mRNA水平比UBQ 4 mRNA水平高3倍，但在花中只有UBQ 4 mRNA水平的三分之二。UBQ 3 mRNA受暗/光处理的调节，而来自UBQ和其他亚型的所有成员的mRNA不受影响。类似地，在UBQ 10/UBQ 11/UBQ 14亚型中，UBQ 11/UBQ 14 mRNA在两小时热激处理后的幼苗中被不同地调节。在所有的多聚泛素基因，UBQ 10 mRNA水平是最恒定的所有器官和环境条件下检查。转UBQ 10基因的转基因植株比转UBQ 3基因的转基因植株具有更高的GUS活性。总之，不同的拟南芥多聚泛素mRNA的相对丰度，即使是那些高度相似的基因在一个亚型内产生的，似乎是调制独立响应于发展和环境的线索。
The highly conserved protein ubiquitin is encoded by five polyubiquitin genes in Arabidopsis thaliana ecotype Columbia that have been divided into two subtypes, the UBQ3/UBQ4 subtype and the UBQ10/UBQ11/UBQ14 subtype. Northern analysis using gene-specific oligonucleotides as hybridization probes and enzyme activity measurements from transgenic plants expressing beta-glucuronidase (GUS) under the control of individual polyubiquitin 5' flanking regions were used to determine the development and environmental regulation of polyubiquitin transcription and mRNA accumulation. Polyubiquitin mRNA levels within and between subtypes were independently modulated. UBQ3 mRNA levels were three-fold higher than UBQ4 mRNA levels in vegetative organs, but only two-thirds of the UBQ4 mRNA levels in flowers. UBQ3 mRNA was modulated by dark/light treatments, while mRNAs from UBQ and all members of the other subtype were unaffected. Similarly, within the UBQ10/UBQ11/UBQ14 subtype, UBQ11/UBQ14 mRNAs were modulated differently in seedlings after a two-hour heat-shock treatment. Among all the polyubiquitin genes, UBQ10 mRNA level was the most constant in all organs and environmental conditions examined. Transgenic plants transformed with a UBQ10 5' flanking region::GUS gene contained higher levels of GUS activity than transgenic plants expressing GUS under the control of UBQ3 5' flanking regions. In conclusion, the relative abundance of different Arabidopsis polyubiquitin mRNAs, even those produced from highly similar genes within a subtype, appears to be modulated independently in response to developmental and environmental cues.