Long-term stability of baculoviruses stored under various conditions.
Long-term stability of baculoviruses stored under various conditions.
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DOI：
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发表时间：
1994-03
期刊：
影响因子：
2.7
通讯作者：
D. Jarvis;A. Garcia
中科院分区：
文献类型：
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作者：
D. Jarvis;A. Garcia
研究了杆状病毒在各种储存条件下的长期稳定性。结果表明，杆状病毒工作储备液在4 ℃下保存数月或一年后，滴度明显降低。粘在塑料管上病毒不会丢失，因为当病毒储存在玻璃管中时也得到了相同的结果。导致病毒感染性丧失的主要因素是暴露于光下。病毒储存在-85度到37度的温度范围内，只要避光，就相当稳定。最后，多次冻融循环未显著降低病毒感染性。这些结果表明，只要避光，杆状病毒工作贮备液可冷冻或冷藏储存，长期感染性损失很小。
The long-term stability of baculoviruses under various storage conditions was investigated. The results showed that the titers of baculovirus working stocks were significantly reduced after the viruses had been stored in polypropylene tubes for several months or a year at 4 degrees C. The virus was not lost by sticking to the plastic tubes, because the same results were obtained when the virus was stored in glass tubes. The major factor contributing to the loss of viral infectivity was exposure to light. Virus stocks stored at temperatures ranging from -85 degrees to 37 degrees C were quite stable, as long as they were protected from light. Finally, viral infectivity was not significantly reduced by multiple freeze-thaw cycles. These results demonstrated that baculovirus working stocks may be stored frozen or refrigerated with little long-term loss of infectivity, as long as they are protected from light.