The SNL-HDA19 histone deacetylase complex antagonizes HY5 activity to repress photomorphogenesis in Arabidopsis
The SNL-HDA19 histone deacetylase complex antagonizes HY5 activity to repress photomorphogenesis in Arabidopsis
复制标题
SNL-HDA19 组蛋白脱乙酰酶复合物拮抗 HY5 活性以抑制拟南芥中的光形态发生
DOI：
10.1111/nph.17114
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发表时间：
2020-12-18
期刊：
影响因子：
9.4
通讯作者：
Lin, Rongcheng
中科院分区：
文献类型：
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作者：
Jing, Yanjun;Guo, Qiang;Lin, Rongcheng
在光形态建成过程中转录组的重编程需要染色质的动态变化和组蛋白修饰的分布。然而，这一过程的染色质为基础的调控仍有待阐明。在这里，我们确定了保守的SWI-INDEPENDENT3 LIKE（SNL）-组蛋白脱乙酰酶19（HDA19）脱乙酰酶复合物，包括HDA19和SNL1-SNL6，作为光信号通路的负调控因子。HDA19和SNL表达的光抑制由光感受器介导。HDA19和SNL是PHYA基因组蛋白去乙酰化和染色质失活所必需的。进一步研究了SNL-HDA 19复合物与伸长下胚轴5（ELONGATED HYPOCOTYL5，HY5）的相互作用及其对靶基因表达的拮抗调控，发现HDA 19脱乙酰酶复合物被HY5募集到两个阳性光信号基因HY5和B-BOX CONTAINING PROTEIN 22（BBX22）的染色质区域，从而降低可接近性和组蛋白乙酰化，抑制其表达。HDA19、SNL1和HY5与HY5和BBX22的相同调控区域相关，并且HY5与这些位点的结合在SNL-HDA19功能障碍时增强。我们的研究揭示了HDA19脱乙酰酶复合物在光信号传导中的关键作用，并表明染色质调节因子和转录因子之间的功能相互作用调节光形态发生对变化的光环境的响应。
Reprogramming of the transcriptome during photomorphogenesis requires dynamic changes in chromatin and distribution of histone modifications. However, the chromatin-based regulation of this process remains to be elucidated.Here, we identify the conserved SWI-INDEPENDENT3 LIKE (SNL)-HISTONE DEACETYLASE19 (HDA19) deacetylase complex, including HDA19 and SNL1-SNL6, as a negative regulator of the light signaling pathway. Light-repression of HDA19 and SNLs expression is mediated by photoreceptors. HDA19 and SNLs are required for histone deacetylation and chromatin inactivation of PHYA gene. We further examined the interaction between SNL-HDA19 complex and ELONGATED HYPOCOTYL5 (HY5), and their antagonistic regulation on the expressions of target genes.The HDA19 deacetylase complex is recruited by HY5 to the chromatin regions of two positive light signaling genes, HY5 and B-BOX CONTAINING PROTEIN 22 (BBX22), thereby reduces the accessibility and histone acetylation and represses their expression. HDA19, SNL1, and HY5 associate with the same regulatory regions of HY5 and BBX22, and HY5 binding to these loci is enhanced upon SNL-HDA19 dysfunction.Our study reveals a crucial role for the HDA19 deacetylase complex in light signaling and demonstrates that the functional interplay between chromatin regulators and transcription factors regulates photomorphogenetic responses to the changing light environments.