OSTEOCLASTIC BONE-RESORPTION BY A POLARIZED VACUOLAR PROTON PUMP
OSTEOCLASTIC BONE-RESORPTION BY A POLARIZED VACUOLAR PROTON PUMP
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DOI：
10.1126/science.2528207
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发表时间：
1989-08-25
期刊：
影响因子：
56.9
通讯作者：
GLUCK, S
中科院分区：
文献类型：
--
作者：
BLAIR, HC;TEITELBAUM, SL;GLUCK, S
骨吸收依赖于破骨细胞形成一个酸性的细胞外腔室，在此腔室内基质被降解。破骨细胞将质子转运到该吸收微环境的机制是通过在分离的破骨细胞膜囊泡中三磷酸腺苷（ATP）依赖的弱碱积累来确定的，这些囊泡表现出液泡型生电的H⁺ -转运三磷酸腺苷酶（H⁺ -ATPase）的底物和抑制特性。通过用从牛肾分离的液泡H⁺ -ATPase抗体对破骨细胞膜蛋白进行免疫印迹，证实了质子泵的同一性。破骨细胞的H⁺ -ATPase通过免疫细胞化学方法定位在细胞 - 骨附着部位。免疫电子显微镜显示H⁺ -ATPase存在于皱褶膜（细胞的吸收器官）中。
Bone resorption depends on the formation, by osteoclasts, of an acidic extracellular compartment wherein matrix is degraded. The mechanism by which osteoclasts transport protons into that resorptive microenvironment was identified by means of adenosine triphosphate-dependent weak base accumulation in isolated osteoclast membrane vesicles, which exhibited substrate and inhibition properties characteristic of the vacuolar, electrogenic H+ -transporting adenosine triphosphatase (H+ -ATPase). Identity of the proton pump was confirmed by immunoblot of osteoclast membrane proteins probed with antibody to vacuolar H+ -ATPase isolated from bovine kidney. The osteoclast''s H+ -ATPase was immunocytochemically localized to the cell-bone attachment site. Immunoelectron microscopy showed that the H+ -ATPase was present in the ruffled membrane, the resorptive organ of the cell.