Role of integrin receptors in manganese-dependent BHK cell spreading on albumin-coated substrata.
Role of integrin receptors in manganese-dependent BHK cell spreading on albumin-coated substrata.
复制标题
整合素受体在锰依赖性 BHK 细胞在白蛋白包被基质上铺展中的作用。
DOI：
10.1016/0014-4827(91)90520-5
复制
发表时间：
1991
影响因子：
3.7
通讯作者：
Backman,R
中科院分区：
文献类型：
--
作者：
Grinnell,F;Backman,R
在含锰的培养液中，组织细胞可以铺展在白蛋白和其他非粘附性底物上，使细胞在含钙/镁的培养液中生长。为了了解整合素受体是否在锰依赖的黏附中起作用，我们检测了RGD多肽和抗纤维连接蛋白受体多克隆抗体对BHK细胞在纤维连接蛋白和白蛋白包裹的底物上铺展的影响。在以纤维连接蛋白为底物的含钙镁介质中，RGD相关肽GRGDSP和GRGDS抑制细胞铺展，而RGDS不抑制细胞铺展。在含锰介质中，GRGDSP和GRGDS以及RGDS对白蛋白的扩散均有抑制作用。另一方面，GRGESP在任何测试条件下都不能抑制细胞扩散。针对纤维连接蛋白受体的抗体也抑制了依赖于锰的细胞在白蛋白底物上的扩散，但需要更高水平的抗体来抑制依赖于钙/镁的纤维连接蛋白的扩散。在这些结果的基础上，我们认为可能是整合素受体，而不是纤维连接蛋白受体，介导了依赖锰的BHK细胞在白蛋白上的扩散。
In manganese-containing medium, tissue cells can spread on albumin and other substrata typically nonadhesive for cells in calcium/magnesium-containing medium. To learn whether integrin receptors play a role in Mn-dependent adhesion, we tested the effects of RGD peptides and polyclonal anti-fibronectin receptor antibodies on BHK cell spreading on fibronectin and albumin-coated substrata. In Ca/Mg-containing medium on fibronectin substrata, the RGD-related peptides GRGDSP and GRGDS but not RGDS inhibited cell spreading. In Mn-containing medium, spreading on albumin was inhibited by GRGDSP and GRGDS and also by RGDS. GRGESP, on the other hand, did not inhibit cell spreading under any condition tested. Antibodies directed against fibronectin receptors also inhibited Mn-dependent cell spreading on albumin substrata, but higher levels of antibody were required than were necessary to inhibit Ca/Mg-dependent spreading on fibronectin. On the basis of these results, we suggest that integrin receptors, but probably not fibronectin receptors, mediate Mn-dependent BHK cell spreading on albumin.