Seizure suppression by adenosine-releasing cells is independent of seizure frequency
Seizure suppression by adenosine-releasing cells is independent of seizure frequency
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DOI：
10.1046/j.1528-1157.2002.33001.x
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发表时间：
2002-08-01
期刊：
影响因子：
5.6
通讯作者：
Möhler, H
中科院分区：
文献类型：
--
作者：
Boison, D;Huber, A;Möhler, H
目的：脑室内细胞递送腺苷最近被证明对大鼠癫痫点燃模型的发作活动有短暂的抑制作用。我们测试了腺苷-re -租赁移植物对癫痫发作的抑制是否与癫痫发作频率无关。方法：将腺苷释放细胞包埋于海马点燃的大鼠侧脑室。在移植后4周内，以每周一次或每周3次的频率进行电刺激试验。通过癫痫发作严重程度的视觉评分和脑电图记录来评估癫痫发作活动。结果：包膜细胞释放的腺苷在大于或等于2周的时间内具有较强的抗癫痫活性。移植后一周，治疗大鼠表现出对阵挛性癫痫发作的完全保护，并且在大多数动物中观察到对局灶性癫痫发作的保护。在脑电图记录中，癫痫发作抑制伴随着放电后减少。无论每周进行1次或3次电刺激，移植细胞的保护效果都是相同的。接受对照移植物的大鼠继续表现出完全的阵挛性抽搐。结论：本研究表明，试验刺激的频率不影响腺苷释放移植物的癫痫抑制电位。因此，局部递送腺苷可能是有效的癫痫发作控制超过三倍的癫痫发作-放电频率范围。
Purpose: Intraventricular cellular delivery of adenosine was recently shown to be transiently efficient in the suppression of seizure activity in the rat kindling model of epilepsy. We tested whether the suppression of seizures by adenosine-re leasing grafts was independent of seizure frequency.Methods: Adenosine-releasing cells were encapsulated and grafted into the lateral brain ventricle of rats kindled in the hippocampus. During 4 weeks after grafting, electric test stimulations were delivered at a frequency of either once a week or 3 times per week. Seizure activity was evaluated by visual scoring of seizure severity and by the recording of EEGs.Results: Adenosine released from encapsulated cells exerted potent antiepileptic activity for greater than or equal to2 weeks. One week after grafting, treated rats displayed a complete protection from clonic seizures, and a protection from focal seizures was observed in the majority of animals. Seizure suppression was accompanied by a reduction of afterdischarges in EEG recordings. The protective efficacy of the grafted cells was the same irrespective of whether electrical test stimulations were delivered 1 or 3 times per week. Rats receiving control grafts continued to display full clonic convulsions.Conclusions: This study demonstrated that the frequency of test stimulations did not influence the seizure-suppressive potential of adenosine-releasing grafts. Thus the local delivery of adenosine is likely to be effective in seizure control over a threefold range of seizure-discharge frequency.