Nucleostemin as a possible progenitor marker of corneal epithelial cells
Nucleostemin as a possible progenitor marker of corneal epithelial cells
复制标题
Nucleostemin 作为角膜上皮细胞可能的祖细胞标记
作者：
M. Kawashima;T. Kawakita;S. Yoshida;S. Shimmura;K. Tsubota
目的 Nucleostemin 是一种参与细胞周期和增殖调节的核蛋白，是多种干细胞的候选标记物。我们检查了 nucleostemin 的表达作为角膜上皮祖细胞分化和衰老的标志物。方法采用RT-PCR检测正常小鼠角膜上皮细胞中Nucleostemin的表达，并采用免疫组织化学法检测培养的小鼠角膜上皮细胞中Nucleostemin的表达。通过免疫组织化学检查与祖细胞标记物 p63 和 Bmi-1、细胞角蛋白 14 和 19、增殖标记物 Ki67、分化标记物细胞角蛋白 12 和外皮蛋白以及与衰老标记物 SA-β-gal 的共表达。还检查了细胞大小和核苷蛋白表达的相关性。结果RT-PCR检测小鼠角膜上皮细胞中Nucleostemin的表达。免疫组织化学显示 nucleostemin 主要在整个角膜的基底细胞和基底上细胞中表达。 nucleostemin 的表达与 Ki67、K14 和 K19 的表达无关，但与 Bmi-1、特别是 p63 的表达相关。 Nucleostemin 不与 SA-β-gal 在同一细胞中共表达。结论 Nucleostemin 可用作类似于 p63 的祖细胞标记。
Purpose Nucleostemin, a nuclear protein involved in the regulation of cell cycle and proliferation, is a candidate marker for various stem cells. We examined the expression of nucleostemin as a marker of differentiation and senescence in corneal epithelial progenitor cells. Methods Nucleostemin expression in normal mouse corneal epithelium was examined by RT-PCR, as well as in cultured mouse corneal epithelial cells by immunohistochemistry. Co-expression with the progenitor markers p63 and Bmi-1, cytokeratin 14 and 19, the proliferating marker Ki67, differentiation markers cytokeratin 12 and involucrin, and with the senescent marker SA-β-gal were examined by immunohistochemistry. Correlation of cellular size and nucleostemin expression was also examined. Results Nucleostemin expression was detected in mouse corneal epithelium by RT-PCR. Immunohistochemistry revealed that nucleostemin was expressed predominantly in basal and suprabasal cells of the whole cornea. The expression of nucleostemin was not associated with the expression of Ki67, K14, and K19, but with the expression of Bmi-1 and particularly with p63. Nucleostemin was not co-expressed with SA-β-gal in same cell. Conclusions Nucleostemin can be used as a progenitor marker analogous to p63.
影响因子：
10.5
作者：
Tsai, RYL;McKay, RDG
通讯作者：
McKay, RDG