Fitness effects of somatic mutations accumulating during vegetative growth
Fitness effects of somatic mutations accumulating during vegetative growth
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DOI：
10.1007/s10682-022-10188-3
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发表时间：
2022-06-04
影响因子：
1.9
通讯作者：
Schwoch，Jaime A.
中科院分区：
文献类型：
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作者：
Cruzan，Mitchell B.;Streisfeld，Matthew A.;Schwoch，Jaime A.
植物独特的生命形式促进了体细胞突变的积累，这些突变可以传递给下一代的后代，因为负责营养生长的相同分生组织细胞也会产生用于有性生殖的配子。然而，很少有人知道的后果体细胞突变积累后代健身。我们评估的健身效果的体细胞突变inMimulus guttatus通过比较后代自花授粉在同一朵花（自花受精），从自花授粉的后代之间的茎在同一植物（geitonogamy）。体细胞突变的影响是显而易见的，从这个比较，自体受精导致纯合性的一部分体细胞突变，但从geitonogamy后代保持杂合的突变独特的每个干。在两个不同的实验中，我们发现一致的健身效果的体细胞突变从个别茎。令人惊讶的是，几个后代群体的自花受精杂交显示出增加的健身相比，从geitonogamy杂交的后代，可能表明，有益的体细胞突变发生在一些茎。这些结果支持了这样的假设，即体细胞突变在营养生长过程中积累，但它们通过在整个发育过程中发生的不同形式的选择进行过滤，导致淘汰表达的有害突变和保留有益突变。
The unique life form of plants promotes the accumulation of somatic mutations that can be passed to offspring in the next generation, because the same meristem cells responsible for vegetative growth also generate gametes for sexual reproduction. However, little is known about the consequences of somatic mutation accumulation for offspring fitness. We evaluate the fitness effects of somatic mutations inMimulus guttatusby comparing progeny from self-pollinations made within the same flower (autogamy) to progeny from self-pollinations made between stems on the same plant (geitonogamy). The effects of somatic mutations are evident from this comparison, as autogamy leads to homozygosity of a proportion of somatic mutations, but progeny from geitonogamy remain heterozygous for mutations unique to each stem. In two different experiments, we find consistent fitness effects of somatic mutations from individual stems. Surprisingly, several progeny groups from autogamous crosses displayed increases in fitness compared to progeny from geitonogamy crosses, likely indicating that beneficial somatic mutations occurred in some stems. These results support the hypothesis that somatic mutations accumulate during vegetative growth, but they are filtered by different forms of selection that occur throughout development, resulting in the culling of expressed deleterious mutations and the retention of beneficial mutations.