INDUCTION INVITRO OF MUCIFICATION OF RAT CUMULUS OOPHORUS BY GONADOTROPINS AND ADENOSINE-3',5'-MONOPHOSPHATE
INDUCTION INVITRO OF MUCIFICATION OF RAT CUMULUS OOPHORUS BY GONADOTROPINS AND ADENOSINE-3',5'-MONOPHOSPHATE
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DOI：
10.1210/endo-102-6-1797
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发表时间：
1978-01-01
期刊：
影响因子：
4.8
通讯作者：
KRAICER, PF
中科院分区：
文献类型：
--
作者：
DEKEL, N;KRAICER, PF
观察了促黄体生成素（LH）在体外诱导排卵前体内细胞间粘液化的能力。该试验旨在测量牛睾丸透明质酸酶（1 mg/ml）溶解卵丘块的能力。分离的卵丘-卵母细胞复合体在LH、FSH [促卵泡激素]、hCG [人绒毛膜促性腺激素]、4种c[环]AMP衍生物之一或3种磷酸二酯酶抑制剂存在下培养24小时。所有这些都显示出能够诱导粘液化。通过每小时检查培养物确定对FSH和LH的粘液化反应的时间。在10 μ g/ml NIH[National Institute of Health]-LH-B 9的存在下，在4小时的潜伏期后出现粘液化;粘液化的最大发生在16小时，半最大发生在6.25小时。在与NIH-FSH-S11孵育的培养物中，这些时间分别为6.0、19.0和9.25 h。LH达到0%、50%和100%应答的时间分别为4.0、6.25和16.0 h，FSH分别为6.0、9.25和19.0 h。从LH的剂量-反应关系的分析中，发现ED 50为2.8 μ g/ml。常规使用的剂量为10 μ g/ml，有效率为88%。卵丘卵母细胞复合体暴露于LH 15分钟，但不是5分钟，足以诱导粘液化反应。卵丘细胞直接对促性腺激素刺激作出反应。它们的反应可能由cAMP介导。
The ability of LH [luteinizing hormone] in vitro to induce the intercellular mucification which occurs in vivo just before ovulation, was observed. The test was to measure the ability of bovine testicular hyaluronidase (1 mg/ml) to lyse the cumulus mass. Isolated cumulus-oocyte complexes were cultured for 24 h in the presence of LH, FSH [follicle stimulating hormone], hCG [human chorionic gonadotropin], 1 of 4 c[cyclic]AMP derivatives, or 3 phosphodiesterase inhibitors. All were shown to be capable of inducing mucification. Timing of the mucification response to FSH and LH was made by examining cultures at hourly intervals. In the presence of 10 .mu.g/ml NIH[National Institute of Health]-LH-B9, mucification appeared after a latent period of 4 h; maximal occurrence of mucification was at 16 h, and half-maximal was at 6.25 h. In cultures incubated with NIH-FSH-S11, these times were 6.0, 19.0, and 9.25 h, respectively. Times for 0%, 50%, and 100% response were 4.0, 6.25, and 16.0 h for LH and 6.0, 9.25, and 19.0 for FSH. From analysis of the dose-response relationship for LH, the ED50 was found to be 2.8 .mu.g/ml. The dose used routinely, 10 .mu.g/ml, was 88% effective. Exposure of cumulus-oocyte complexes to LH for 15, but not 5, min sufficed for the induction of the mucification response. Cumulus cells respond directly to gonadotrophic stimulus. Their response is presumably mediated by cAMP.