Plasma membrane lipid packing and leukocyte function-associated antigen-1-dependent aggregation of lymphocytes.
Plasma membrane lipid packing and leukocyte function-associated antigen-1-dependent aggregation of lymphocytes.
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质膜脂质堆积和白细胞功能相关的抗原 1 依赖性淋巴细胞聚集。
DOI：
10.1002/jcp.1041560124
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发表时间：
1993
影响因子：
5.6
通讯作者：
Schlegel,RA
中科院分区：
文献类型：
--
作者：
Smith,DM;Williamson,PL;Schlegel,RA
研究白细胞功能相关抗原1（LFA-1）介导的粘附的一个简单模型系统是体外刺激的淋巴细胞聚集。尽管LFA-1的单克隆抗体可以阻断聚集，但并非所有表达LFA-1的淋巴细胞都聚集，这表明LFA-1是聚集所必需的，但并不足以聚集。为了研究脂质双层是否在LFA-1的功能活化中起作用，在培养物中刺激人外周血淋巴细胞和小鼠脾淋巴细胞，并测量质膜脂质的聚集与堆积。细胞向聚集体的进展通过总体群体的脂质堆积的减少来抑制，如通过用荧光探针mercury 540增加染色来监测的。来自聚集体的细胞比来自相同群体的非聚集细胞染色更强烈，表明聚集体优先由具有疏松包装膜的群体中的细胞形成。相比之下，发现聚集细胞表达与非聚集细胞相等或甚至更低量的LFA-1。因此，松散的脂质包装与LFA-1依赖性聚集的发展相关，并可能参与这种细胞粘附分子的功能激活。© 1993 Wiley利斯公司
A simple model system for study of adhesion mediated by leukocyte function‐associated antigen‐1 (LFA‐1) is aggregation of lymphocytes stimulated in vitro. Although aggregation is blocked by monoclonal antibodies to LFA‐1, not all lymphocytes expressing LFA‐1 aggregate, indicating that LFA‐1 is necessary but not sufficient for aggregation. To investigate whether the lipid bilayer plays a role in the functional activation of LFA‐1, human peripheral blood lymphocytes and murine splenic lymphocytes were stimulated in culture, and measurements made of aggregation vs. packing of plasma membrane lipids. Progression of cells into aggregates was paralleled by a decrease in lipid packing of the population as a whole, as monitored by increased staining with the fluorescent probe merocyanine 540. Cells from aggregates stained more intensely than nonaggregated cells from the same population, indicating that aggregates are preferentially formed from cells in the population with the loosest packed membrane. In contrast, aggregated cells were found to express equivalent or even lower amounts of LFA‐1 than nonaggregated cells. Looser lipid packing is therefore associated with the development of LFA‐1‐dependent aggregation, and might be involved in the functional activation of this cell adhesion molecule. © 1993 Wiley‐Liss, Inc.