Protective effects of baicalin on LPS-induced injury in intestinal epithelial cells and intercellular tight junctions
Protective effects of baicalin on LPS-induced injury in intestinal epithelial cells and intercellular tight junctions
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黄芩苷对脂多糖诱导的肠上皮细胞及细胞间紧密连接损伤的保护作用
DOI：
10.1139/cjpp-2014-0262
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发表时间：
2015-04-01
影响因子：
2.1
通讯作者：
Kuang, Zaoyuan
中科院分区：
文献类型：
--
作者：
Chen, Jian;Zhang, Ren;Kuang, Zaoyuan
目的：探讨黄芩苷对内毒素诱导的肠上皮细胞和细胞间紧密连接损伤的保护作用及其机制。方法：用含或不含黄芩苷的内毒素(1.0 mU g/m L)刺激IEC-6细胞24 h，用双抗体夹心法测定细胞内炎性细胞因子IL-6和肿瘤坏死因子-α(TNF-α)水平。用实时定量聚合酶链式反应检测Claudin-3、occludin和ZO-1的mRNA表达水平，用Western印迹和免疫荧光分析ZO-1蛋白的表达水平和分布模式。结果：黄芩苷(10.0微克/毫升)可提高脂多糖刺激的细胞存活率，抑制IL-6和TNF-α水平。此外，在脂多糖损伤的IEC-6细胞中，黄芩苷可上调ZO-1的mRNA和蛋白表达水平，并维持其蛋白的完整表达。结论：黄芩苷对内毒素损伤的IEC-6细胞及细胞间紧密连接具有保护作用。其作用机制可能与抑制炎性细胞因子的产生，上调ZO-1的mRNA和蛋白表达有关。
Aims: To investigate the protective effects and mechanisms of baicalin on lipopolysaccharide (LPS)-induced injury in intestinal epithelial cells and intercellular tight junctions. Methods: IEC-6 cells were stimulated with LPS (1.0 mu g/mL), with or without baicalin, for 24 h. The levels of the inflammatory cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha were determined using ELISA. Quantitative real-time PCR was used for determining the mRNA expression level of claudin-3, occludin, and ZO-1; Western blot and immunofluorescence analysis were used for analyzing the expression level and the distribution patterns of ZO-1 protein. Results: Pretreatment with baicalin (10.0 mu g/mL) improved LPS-stimulated cell viability and repressed IL-6 and TNF-alpha levels. In addition, pretreatment with baicalin up-regulated mRNA and protein expression levels of ZO-1 and kept the protein intact in IEC-6 cells injured with LPS. Conclusion: Baicalin has the capacity to protect IEC-6 cells and the intercellular tight junctions from LPS-induced injury. The mechanisms may be associated with inhibiting the production of inflammatory cytokines, and up-regulating the mRNA and protein expression of ZO-1.