Plasticity of astroglia: evidence supporting process elongation by "stretch".
Plasticity of astroglia: evidence supporting process elongation by "stretch".
复制标题
星形胶质细胞的可塑性：支持“拉伸”过程伸长的证据。
作者：
Jaeger,CB
通过在体植入实验研究了脑星形胶质细胞的结构可塑性。胶质丝蛋白免疫细胞化学标记用于鉴定星形胶质细胞。首先确定植入的硝酸纤维素过滤器为来自年轻大鼠不同脑区的星形胶质细胞提供了基质。星形胶质细胞贴附于滤器表面，并向滤器内投射细小的突起，植入时间越长，滤器内胶质突起的密度和长度越大。星形胶质细胞穿透植入在顶盖中的过滤器表现出更多的过程比那些在皮质中，表明星形胶质细胞分布的区域差异。第二，观察到当星形胶质细胞被拴系在膨胀的基质内时，附着于过滤器的星形胶质细胞形成伸长的突起。这通过将硝酸纤维素过滤器与继续生长的PC 12细胞一起植入来显示。无论是植入PC 12细胞没有过滤器，也没有硝酸纤维素过滤器单独诱导形成细长的星形胶质细胞与平行排列的过程，类似放射状胶质细胞。这种胶质细胞形式仅发生在滤器/PC 12细胞共移植物中。这表明粘附于硝酸纤维素滤器的星形胶质细胞的突起可以响应于周围组织的扩张而拉伸。
The structural plasticity of cerebral astroglia was investigated in vivo by implantation experiments. Immunocytochemical markers for glia filament protein were used to identify the astrocytes. First it was established that implanted nitrocellulose filters provided a substrate for astrocytes from different brain regions of young rats. Astrocytes attached to the filter and projected fine processes into it. Longer implantation times increased the density and length of glial processes within filter spaces. Astrocytes that penetrated the filters implanted in the tectum exhibited more processes than those in the cortex, suggesting regional differences of astrocyte distributions. Second it was observed that astrocyted that attached to the filter formed elongated processes when they were tethered within an expanding matrix. This was shown by implanting the nitrocellulose filter together with PC12 cells that continued to grow. The implantation of neither PC12 cells without filters nor nitrocellulose filters alone induced the formation of elongated astroglia with parallel aligned processes, resembling radial glia. Such glial forms only occurred in the filter/PC12 cell cografts. This indicates that processes of astrocytes adherent to nitrocellulose filters could be stretched in response to expansion of the surrounding tissue.