Sucrose transporter StSUT4 from potato affects flowering, tuberization, and shade avoidance response
Sucrose transporter StSUT4 from potato affects flowering, tuberization, and shade avoidance response
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DOI：
10.1104/pp.107.112334
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发表时间：
2008-02-01
期刊：
影响因子：
7.4
通讯作者：
Kuehn, Christina
中科院分区：
文献类型：
--
作者：
Chincinska, Izabela A.;Liesche, Johannes;Kuehn, Christina
蔗糖转运蛋白（Suc）属于一个大的基因家族。SUT 1蛋白在高等植物中的生理作用已被深入研究，而SUT 4蛋白的生理作用迄今尚不清楚。马铃薯（Solanum tuberosum）的所有三种已知Suc转运蛋白SUT 1、SUT 2和SUT 4共定位，它们的RNA水平不仅遵循昼夜节律，而且在恒定光照下振荡。在这里，我们研究了转基因马铃薯植物对RNA干扰（RNAi）失活的StSUT 4表达的生理效应。StSUT 4-RNAi植物的表型包括早花、较高的块茎产量和对富含远红光波长的光的敏感性降低（即，e.在树冠阴影中）。StSUT 4的抑制导致了严格光周期马铃薯亚种的块茎生产。andigena即使在非感应长日条件下。可溶性糖的积累和转基因植物叶片的Suc流出在StSUT 4-RNAi植物中被修饰，导致库器官中的Suc水平被修饰。野生型植物的StSUT 4表达由赤霉素和乙烯利诱导，并且赤霉素的外部供应导致野生型和StSUT 4-RNAi植物之间关于块茎产量和节间伸长的甚至更显著的差异，表明StSUT 4和赤霉素的相互调节。
Sucrose (Suc) transporters belong to a large gene family. The physiological role of SUT1 proteins has been intensively investigated in higher plants, whereas that of SUT4 proteins is so far unknown. All three known Suc transporters from potato (Solanum tuberosum), SUT1, SUT2, and SUT4, are colocalized and their RNA levels not only follow a diurnal rhythm, but also oscillate in constant light. Here, we examined the physiological effects of transgenic potato plants on RNA interference (RNAi)inactivated StSUT4 expression. The phenotype of StSUT4-RNAi plants includes early flowering, higher tuber production, and reduced sensitivity toward light enriched in far-red wavelength (i. e. in canopy shade). Inhibition of StSUT4 led to tuber production of the strict photoperiodic potato subsp. andigena even under noninductive long-day conditions. Accumulation of soluble sugars and Suc efflux from leaves of transgenic plants are modified in StSUT4-RNAi plants, leading to modified Suc levels in sink organs. StSUT4 expression of wild-type plants is induced by gibberellins and ethephon, and external supply of gibberellic acid leads to even more pronounced differences between wild-type and StSUT4-RNAi plants regarding tuber yield and internode elongation, indicating a reciprocal regulation of StSUT4 and gibberellins.