Efficacy and Safety of an Oral Somatostatin DNA Vaccine without Antibiotic Resistance Gene in Promoting Growth of Piglets
Efficacy and Safety of an Oral Somatostatin DNA Vaccine without Antibiotic Resistance Gene in Promoting Growth of Piglets
复制标题
口服不含抗生素抗性基因的生长抑素DNA疫苗促进仔猪生长的有效性和安全性
DOI：
10.1111/sji.12155
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发表时间：
2014-04-01
影响因子：
3.7
通讯作者：
Yang, L. -G.
中科院分区：
文献类型：
--
作者：
Han, Y. -G.;Liang, A. -X.;Yang, L. -G.
本研究旨在评价无耐药基因的减毒霍乱沙门氏菌C500介导的抗生长抑素（SS） （pGS/2SS-asd，编码2拷贝生长抑素基因）口服DNA疫苗对仔猪生长的有效性和安全性。50头仔猪均匀分为5组。前三组动物口服5 × 10（10）、5 × 10(9)或5 × 10(8)菌落形成单位（CFU）剂量的疫苗。其余两组分别口服细菌C500（含不含生长抑素基因的pVAX-asd质粒）或磷酸盐缓冲盐水（PBS）作为对照。结果表明，疫苗诱导ss特异性抗体呈剂量依赖性。与PBS对照组相比，高剂量组动物血清SS水平较低，生长激素（GH）水平较高。高剂量组4周和8周平均日增重分别提高32.88%和26.46%。抗ss抗体与初次免疫后第8周生长激素水平或平均日增重呈正相关(P
This study aimed to evaluate the efficacy and safety of an oral DNA vaccine against somatostatin (SS) (pGS/2SS-asd, encoding two copies of somatostatin genes) mediated by attenuated Salmonella choleraesuis C500 without antibiotic resistance gene on piglets growth. A total of 50 piglets were uniformly divided into five groups. The animals in the first three groups were orally given vaccine in dose of either 5x10(10), 5x10(9) or 5x10(8) colony-forming units (CFU). The remaining two groups were orally administered with either bacteria C500 (containing pVAX-asd plasmid without somatostatin gene) or phosphate-buffered saline (PBS) as controls. The results indicated that the vaccine induced SS-specific antibodies in a dose-dependent pattern. Compared with the PBS control, animals in the high-dose group showed lower SS levels and higher growth hormone (GH) levels in sera. Average daily gain of animals in the high-dose group was increased by 32.88% and 26.46% during 4 and 8weeks, respectively. Anti-SS antibodies were positively correlated with either GH levels or average daily gain at week 8 after primary immunization (P