Amplified Fragment Length Polymorphism (AFLP) Provides Molecular Markers for the Identification of Caladium bicolor Cultivars
Amplified Fragment Length Polymorphism (AFLP) Provides Molecular Markers for the Identification of Caladium bicolor Cultivars
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DOI：
10.1006/anbo.1999.0903
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发表时间：
1999-08
期刊：
影响因子：
4.2
通讯作者：
J. Loh;R. Kiew;Andrea Kee;L. Gan;Y. Gan
中科院分区：
文献类型：
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作者：
J. Loh;R. Kiew;Andrea Kee;L. Gan;Y. Gan
摘要 贝母是流行的观赏植物，但在分子水平上尚未得到充分研究。花芋属（天南星科）内的物种鉴定主要基于形态学。然而，由于缺乏全面的参考文献，使得花芋品种的鉴定变得极其困难。使用 17 个引物组合对两种花芋 (C. bicolor 和 C. schomburgkii) 进行了扩增片段长度多态性 (AFLP) 分析，其中包括 6 个花芋品种。结果表明，AFLP 可用于通过这两个物种独特且不同的条带图案来区分它们。使用算术平均的未加权配对方法 (UPGMA) 允许对来自 6 个双色 C. bicolor 品种和一个 C. schomburgkii 品种的 17 个选定引物组合的数据进行聚类分析。这表明可以清楚地区分密切相关的物种，并且也可以建立品种之间的遗传差异。所有使用的 C. bicolor 品种均检测到独特的 AFLP 分子标记。 AFLP 的使用有可能精确表征和识别特定的贝母品种以及注册新品种。它也将有助于未来的育种计划和系统学研究。
Abstract Caladiums are popular ornamental plants that have not been well studied at the molecular level. Identification of species within the genus Caladium (Araceae) has been based primarily on morphology. However, the lack of comprehensive references makes identification of Caladium cultivars extremely difficult. Amplified fragment length polymorphism (AFLP) analysis using 17 primer combinations was carried out on two species of Caladium ( C. bicolor and C. schomburgkii ), including six cultivars of C. bicolor . Results showed that AFLP can be used to distinguish these two species by their unique and different banding patterns. Unweighted Pair Group Method using Arithmetic Averages (UPGMA) permitted cluster analysis of data from 17 selected primer combinations on six cultivars of C. bicolor and one cultivar of C. schomburgkii . It showed that closely related species can clearly be differentiated and that genetic difference between cultivars can also be established. Unique AFLP molecular markers were detected for all the C. bicolor cultivars used. The use of AFLP has potential for precisely characterizing and identifying particular caladium cultivars as well as for the registration of new cultivars. It will also be useful in future breeding programmes and systematics studies.