Progesterone stimulation of fluid absorption by the rat uterine gland
Progesterone stimulation of fluid absorption by the rat uterine gland
复制标题
DOI：
10.1530/rep.0.1230633
复制
发表时间：
2002-05-01
期刊：
影响因子：
3.8
通讯作者：
Milligan, SR
中科院分区：
文献类型：
--
作者：
Naftalin, RJ;Thiagarajah, JR;Milligan, SR
本研究的目的是检验类固醇环境影响子宫腺液体吸收的假设。激光扫描共聚焦显微镜的细胞外标记物（异硫氰酸荧光素标记的葡聚糖）在大鼠子宫腺内的分布表明，子宫内膜腺体改变其流体处理特性在不同的激素条件下。在孕酮主导下，腺体表现出阿米洛利敏感的葡聚糖积累，表明钠依赖性液体吸收;然而，这是不存在的雌激素为主的状态。在孕酮刺激的腺体开口中的液体摄取速率估计约为1 x 10（-4）cm s（-1），需要在粘膜表面处的抽吸压力在10和20 mm Hg之间。这项研究提供了子宫腺吸收液体的第一个直接证据。这种吸收可能提供了子宫腔闭合和胚泡固定的机制，这是着床所必需的。
The purpose of this study was to test the hypothesis that the steroid environment affects fluid absorption by the uterine glands. Laser scanning confocal microscopy of the distribution of an extracellular marker (fluorescein isothiocyanate-labelled dextran) within rat uterine glands showed that the endometrial glands change their fluid handling characteristics under different hormonal conditions. Under progesterone dominance, the glands showed an amiloride-sensitive dextran accumulation indicating sodium-dependent fluid absorption; however, this was absent in the oestrogen-dominated state. The rate of fluid uptake in the progesterone-stimulated gland opening was estimated to be approximately 1 x 10(-4) cm s(-1), requiring a suction pressure of between 10 and 20 mm Hg at the mucosal surface. This study provides the first direct evidence of fluid absorption by the uterine glands. Such absorption may provide the mechanism for closure of the uterine lumen and immobilization of the blastocyst necessary for implantation.