A serine/threonine phosphatase 1 of Streptococcus suis type 2 is an important virulence factor.
A serine/threonine phosphatase 1 of Streptococcus suis type 2 is an important virulence factor.
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猪链球菌2型丝氨酸/苏氨酸磷酸酶1是重要的毒力因子
DOI：
10.4142/jvs.2017.18.4.439
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发表时间：
2017-12-31
影响因子：
1.8
通讯作者：
Fang W
中科院分区：
文献类型：
--
作者：
Fang L;Zhou J;Fan P;Yang Y;Shen H;Fang W
猪链球菌被认为是猪的主要病原体之一，而猪链球菌2型(SS2)被认为是一种人畜共患病细菌，它能引起人类脑膜炎和链球菌中毒性休克综合征。许多细菌物种都含有编码丝氨酸/苏氨酸蛋白磷酸酶(STP)的基因，该基因负责在单一反应步骤中使其底物去磷酸化。本研究探讨了stp1在SS2发病机制中的作用。从Δ菌株ZJ081101中构建了一个等基因的stp1突变株(SS1stp1)。与亲本株相比，Δstp1突变体显著增加了对HEp-2和bEnd.3细胞的黏附，并提高了RAW264.7细胞的存活率。巨噬细胞存活率的提高可能与对活性氧的抵抗有关，因为Δstp1突变体比其亲本菌株对百草枯诱导的氧化应激具有更强的抵抗力。然而，与亲本菌株的毒力相比，stp1的缺失显著减弱了SS2对小鼠的毒力，在小鼠模型中，其致死剂量50值几乎是亲本的两倍，器官和血液中的细菌载量较低。我们得出结论，Stp1在SS2毒力中起着重要作用。
Streptococcus suis is regarded as one of the major pathogens of pigs, and Streptococcus suis type 2 (SS2) is considered a zoonotic bacterium based on its ability to cause meningitis and streptococcal toxic shock-like syndrome in humans. Many bacterial species contain genes encoding serine/threonine protein phosphatases (STPs) responsible for dephosphorylation of their substrates in a single reaction step. This study investigated the role of stp1 in the pathogenesis of SS2. An isogenic stp1 mutant (Δstp1) was constructed from SS2 strain ZJ081101. The Δstp1 mutant exhibited a significant increase in adhesion to HEp-2 and bEnd.3 cells as well as increased survival in RAW264.7 cells, as compared to the parent strain. Increased survival in macrophage cells might be related to resistance to reactive oxygen species since the Δstp1 mutant was more resistant than its parent strain to paraquat-induced oxidative stress. However, compared to parent strain virulence, deletion of stp1 significantly attenuated virulence of SS2 in mice, as shown by the nearly double lethal dose 50 value and the lower bacterial load in organs and blood in the murine model. We conclude that Stp1 has an essential role in SS2 virulence.
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影响因子：
1.8
作者：
Haas B;Vaillancourt K;Bonifait L;Gottschalk M;Grenier D
通讯作者：
Grenier D
影响因子：
4.2
作者：
Martinez MA;Das K;Saikolappan S;Materon LA;Dhandayuthapani S
通讯作者：
Dhandayuthapani S
影响因子：
4.8
作者：
Archambaud, Cristel;Nahori, Marie-Anne;Dussurget, Olivier
通讯作者：
Dussurget, Olivier
影响因子：
3.2
作者：
Ju, Cun-Xiang;Gu, Hong-Wei;Lu, Cheng-Ping
通讯作者：
Lu, Cheng-Ping
影响因子：
--
作者：
Grenier, Daniel;Bodet, Charles
通讯作者：
Bodet, Charles