ISOLATION OF MARBURG-LIKE VIRUS FROM A CASE OF HÆMORRHAGIC FEVER IN ZAIRE
ISOLATION OF MARBURG-LIKE VIRUS FROM A CASE OF HÆMORRHAGIC FEVER IN ZAIRE
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DOI：
10.1016/s0140-6736(77)92002-5
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发表时间：
1977-03
期刊：
影响因子：
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通讯作者：
S. Pattyn;G. Groen;W. Jacob;P. Piot;G. Courteille
中科院分区：
文献类型：
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作者：
S. Pattyn;G. Groen;W. Jacob;P. Piot;G. Courteille
这三篇论文都描述了从一名扎伊尔患者的血液中分离出一种病毒的情况，该患者在1976年下半年扎伊尔北部和苏丹南部大规模爆发出血热期间患病。来自扎伊尔的材料最初被送到安特卫普，然后从那里的标本被送到波顿唐恩和亚特兰大。在豚鼠和Vero细胞培养中分离出了原型病毒，但在小鼠身上的分离尝试没有结果。豚鼠出现发热性疾病，同时在Vero细胞中检测到一些细胞病变和胞浆内包涵体。3例死后人肝标本和接种该病毒的豚鼠肝组织病理学检查显示，肝细胞局灶性坏死，完整肝细胞内有大量大片嗜酸粒细胞包涵体。豚鼠的脾和淋巴结表现为广泛的淋巴组织耗竭。电子显微镜研究发现，肝脏中有许多丝状病毒颗粒。在Vero细胞培养中也发现了类似的颗粒。它们的直径约为100 nm，长度从300 nm到1500 nm不等，通常带有末端气泡。有几张优秀的电子显微镜照片说明了作者的观点，即这些颗粒在形态上与1967年在德国和1975年在南非分离的马尔堡病毒株难以区分。然而，Johnsonet等人的论文描述了比较马尔堡病毒和扎伊尔病毒的荧光抗体研究。检测到明显的抗原性差异。Bowenet等人报告从扎伊尔和苏丹分离到另外3株病毒株，而Johnsonet等人发现苏丹2名患者的早期康复血清中含有对扎伊尔病毒的抗体，但不含有对马尔堡病毒的抗体。新的毒株DI.H.辛普森已被提议命名为埃博拉病毒。
These three papers all describe the isolation and characterization of a virus from the blood of a Zairian patient who became ill during a large outbreak of haemorrhagic fever in northern Zaire and southern Sudan in the second half of 1976. Material from Zaire was originally sent to Antwerp and from there specimens were dispatched to Porton Down and Atlanta.The prototype virus was isolated in guineapigs and Vero cell cultures but isolation attempts in mice were inconclusive. Guineapigs developed a febrile illness while some cytopathic effects and intracytoplasmic inclusions were detected in Vero cells. Histopathological examination of 3 post-mortem human liver specimens and livers from guineapigs inoculated with the prototype virus showed focal hepatocellular necrosis and many large eosinophilic inclusions in intact hepatocytes. Guineapig spleen and lymph nodes showed widespread depletion of lymphoid tissue. Electron microscopic studies revealed many filamentous virus particles in the liver. Similar particles were found in Vero cell cultures. Their diameter was approximately 100 nm and their length varied from 300 nm to 1500 nm, often with terminal blebs. There are several excellent electronmicrographs illustrating the authors' opinion that the particles are morphologically indistinguishable from the Marburg virus strains isolated in Germany in 1967 and South Africa in 1975. The paper by Johnsonet al., however, describes fluorescent antibody studies comparing Marburg virus with the Zaire virus. A distinct antigenic difference was detected. Bowenet al.report the isolation of 3 further virus strains from Zaire and 1 from Sudan while Johnsonet al.found that early convalescent sera from 2 patients in Sudan contained antibody to the Zaire virus but not to Marburg. The name Ebola virus has been proposed for the new strains,D. I. H. Simpson.