Extensive analysis of EST sequences reveals that all cichlid species in Lake Victoria share almost identical transcript sets
Extensive analysis of EST sequences reveals that all cichlid species in Lake Victoria share almost identical transcript sets
复制标题
DOI：
10.1016/j.gene.2008.11.023
复制
发表时间：
2009-07-15
期刊：
影响因子：
3.5
通讯作者：
Okada, Norihiro
中科院分区：
文献类型：
--
作者：
Kobayashi, Naoki;Watanabe, Masakatsu;Okada, Norihiro
维多利亚湖拥有数百种慈鲷，它们在进化过程中积极发展其形态特征。特别是，颌骨和牙齿的形状已经明显不同，并与摄食生态学相关。这些物种被认为是在过去的15,000年里爆炸性地出现的，因此对进化生物学家特别感兴趣。在此之前，我们报道了两种慈鲷的表达序列标签（EST），Haplochromis chilotes和H。sp.“redtailsheller”的EST序列进行了比较分析。在这项研究中，我们进一步研究了维多利亚湖慈鲷多样性的分子基础，通过产生EST从另一个慈鲷物种，H。sp.“Matumbi hunter”; 9，219个克隆用于序列测定，从该EST集中新获得16，795个序列。比较分析的68个基因共同的三种慈鲷发现，遗传距离的程度和遗传多态性的程度是高度相似的每一个成对的比较，这表明蛋白质编码区的三个慈鲷基因组是基本相同的。由于用于比较分析的三个物种在形态和生态特征上特别不同，我们得出结论，维多利亚湖慈鲷属物种之间的转录本序列的一致性是一个全球性的特征。(C)2008 Elsevier B. V.保留所有权利。
Lake Victoria harbors hundreds of cichlid species, which have aggressively developed their morphological features through their evolution. In particular, the shapes of jaws and teeth have diverged markedly, and correlate with feeding ecology. These species are believed to have explosively arisen within the last 15,000 years and are therefore of particular interest to evolutionary biologists. Previously, we reported expressed sequence tags (ESTs) from two cichlid species, Haplochromis chilotes and H. sp. "redtailsheller", and provided a comparative EST analysis. In this study, we further examined the molecular basis for diversification of Lake Victoria cichlids by generating EST from another cichlid species, H. sp. "Matumbi hunter"; 9,219 clones were applied for sequence determination and 16,795 sequences were newly obtained from this EST set. Comparative analysis of the 68 genes common to the three cichlid species revealed that the degrees of genetic distance and the degrees of genetic polymorphism were highly similar for each pair-wise comparison, suggesting that the protein coding regions of the three cichlid genomes are essentially the same. Because the three species used for the comparative analysis were particularly diverged from each other in morphological and also ecological features, we concluded that the uniformity of transcript sequences among cichlid species in Lake Victoria is a global feature. (C) 2008 Elsevier B.V. All rights reserved.