Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni.
Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni.
复制标题
空肠弯曲杆菌高亲和力铁获取系统的表征。
DOI：
10.1007/978-1-4939-6536-6_7
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Lin,Jun
中科院分区：
文献类型：
--
作者：
Zeng,Ximin;Lin,Jun
铁获取系统对细菌的发病机制至关重要，因此已被提出作为铁依赖性病原体控制的有吸引力的靶点。在这些系统中，铁载体（一种小型铁螯合剂）介导的高亲和力铁获取是革兰氏阴性菌中最有效的铁清除机制。弯曲杆菌不产生任何铁载体，但具有利用外源铁载体的能力。特别是肠杆菌（enterobactin, Ent）介导的铁清除与弯曲杆菌的发病密切相关。迄今为止，Ent是一种已知与铁亲和力最高的三儿茶酚酸，是弯曲杆菌在体内感染过程中用于铁获取的一种特性良好的铁载体。在这里，我们描述了用于表征ent介导的空肠弯曲杆菌高亲和力铁获取系统的关键方法。
Iron acquisition systems are critical for bacterial pathogenesis and thus have been proposed as attractive targets for iron-dependent pathogen control. Of these systems, high-affinity iron acquisition mediated by siderophore, a small iron chelator, is the most efficient iron-scavenging mechanism in gram-negative bacteria.Campylobacterdoes not produce any siderophores but has the ability to utilize exogenous siderophores. In particular, the enterobactin (Ent)-mediated iron scavenging is tightly linked toCampylobacterpathogenesis. To date, Ent, a triscatecholate with the highest known affinity for ferric iron, is a well-characterized siderophore used byCampylobacterfor iron acquisition during in vivo infection. Here, we describe the key methods used to characterize Ent-mediated high affinity iron acquisition system inCampylobacter jejuni.