Direct parabrachial nuclear projections to the pharyngeal motoneurons in the rat: an anterograde and retrograde double-labeling study
Direct parabrachial nuclear projections to the pharyngeal motoneurons in the rat: an anterograde and retrograde double-labeling study
复制标题
大鼠咽部运动神经元的直接臂旁核投射：一项顺行和逆行双标记研究
DOI：
10.1016/s0006-8993(98)01127-5
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发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
M. Seki
中科院分区：
文献类型：
--
作者：
T. Hayakawa;Jun;M. Seki
臂旁核由几个亚核组成，含有自主神经、味觉、内脏感觉、伤害感受和呼吸相关神经元。我们用顺行和逆行双示踪技术研究了大鼠臂旁区（包括Kölliker-Nerve核）到咽运动神经元的直接投射。将生物素化葡聚糖胺注射到臂旁核腹外侧区（包括臂旁中央外侧核的外内侧、外外侧和新月形区）和Köliker-Ehrman核后，将霍乱毒素亚基B注射到下咽或食管中。这些核团的葡聚糖胺顺行标记纤维除投射到疑核致密结构外，还投射到半致密结构、疏松结构和外部结构。在疑核的半致密结构中，许多顺标纤维和终末逆行接触霍乱毒素标记的咽神经元索马胞体和树突。臂旁核的内侧半部分，包括中央外侧臂旁核的内侧和内侧部分，向食管运动神经元背侧的网状结构发送了一些纤维，但没有向咽部或食管运动神经元发送纤维。这些结果提示，臂旁核内内脏感觉、味觉、伤害性或呼吸相关神经元直接投射到咽运动神经元，而疑核内没有向食管运动神经元的臂旁投射。
The parabrachial nucleus consists of several subnuclei which contains autonomic, gustatory, visceral sensory, nociceptive, and respiratory related neurons. We have investigated the direct projections from the rat parabrachial region, including the Kölliker–Fuse nucleus, to the pharyngeal motoneurons with an anterograde and retrograde double-tracing technique. The cholera toxin subunit-B was injected into the lower pharynx or the esophagus after injection of biotinylated dextran amine into the ventrolateral parabrachial nuclear region, including the external medial, the external lateral, and the crescent area of the central lateral parabrachial nuclei and into the Kölliker–Fuse nucleus. The anterogradely dextran amine-labeled fibers from these nuclei projected to the semicompact, loose and external formations besides the compact formation of the nucleus ambiguus. Many anterogradely labeled fibers and terminals were found to contact retrogradely cholera toxin-labeled pharyngeal neuronal soma and dendrites in the semicompact formation of the nucleus ambiguus. The medial half of the parabrachial nucleus, including the medial and the medial part of the central lateral parabrachial nuclei, sent a few fibers to the reticular formation just dorsal to the esophageal motoneurons but no fibers to either the pharyngeal or to the esophageal motoneurons. These results suggested that the visceral sensory, gustatory, nociceptive or respiratory related neurons in the parabrachial nucleus project directly to the pharyngeal motoneurons, but there are no parabrachial projections to the esophageal motoneurons in the nucleus ambiguus.