Population Differentiation in the Pacific White-sided Dolphin Lagenorhynchus obliquidens Inferred from Mitochondrial DNA and Microsatellite Analyses
Population Differentiation in the Pacific White-sided Dolphin Lagenorhynchus obliquidens Inferred from Mitochondrial DNA and Microsatellite Analyses
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DOI：
10.2108/zsj.21.989
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发表时间：
2004-09
期刊：
影响因子：
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通讯作者：
A. Hayano;M. Yoshioka;Miho Tanaka;M. Amano
中科院分区：
文献类型：
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作者：
A. Hayano;M. Yoshioka;Miho Tanaka;M. Amano
摘要通过对线粒体DNA和核微卫星变异的分析，研究了日本近海和北太平洋近海太平洋白边海豚的遗传多样性和分化。对59个个体的线粒体控制区的519个碱基进行了测序，并对5个微卫星座位进行了基因分型。高水平的单倍型多样性(h=96.1%)，中等水平的核苷酸多样性(π=1.65%)和平均期望杂合度(HE=0.66-0.76)都在其他齿形动物的报道范围内。通过基于mtDNA和微卫星变异的分子方差分析、遗传变异比较以及mtDNA单倍型和微卫星等位基因的地理分布，表明日本太平洋沿岸和北太平洋近海样品之间存在一致的遗传差异。这一结果表明，上述两个地区的太平洋白边海豚都属于不同的种群，它们之间的基因流动受到了严重限制。日本近海种群的低遗传多样性和mtDNA谱系表明，它起源于日本海的一个小种群，或者在晚更新世冰期将日本海与北太平洋隔绝时经历了种群减少。
Abstract We investigated genetic diversity and differentiation of the Pacific white-sided dolphin (Lagenorhynchus obliquidens) in Japanese coastal waters and offshore North Pacific by analyzing mitochondrial DNA and nuclear microsatellite variation. A total of 519 bp of the mitochondrial control region was sequenced and five microsatellite locus were genotyped for 59 individuals. A high level of haplotypic diversity (h=96.1%), moderate level of nucleotide diversity (π=1.65%) and average expected heterozygosity (HE=0.66–0.76) were within an extent of those reported for other odontocetes. Consistent genetic difference between the samples from Japanese coastal Pacific-Sea of Japan and offshore North Pacific was indicated by analyses of molecular variance (AMOVAs) based on mtDNA and microsatellite variations, comparison of genetic variabilities, and geographical distributions of mtDNA haplotypes and microsatellite alleles. This result suggests that Pacific white-sided dolphins in each of the above two areas belong to different populations between which gene flow has been severely restricted. The low genetic diversity and mtDNA genealogy of the population in Japanese coastal waters suggest that it originated from a small population that colonized the Sea of Japan or that experienced population reduction when this Sea was isolated from the North Pacific during a glacial period in the Late Pleistocene.