BCL6 gene translocation in follicular lymphoma:: a harbinger of eventual transformation to diffuse aggressive lymphoma
BCL6 gene translocation in follicular lymphoma:: a harbinger of eventual transformation to diffuse aggressive lymphoma
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DOI：
10.1182/blood-2002-08-2482
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发表时间：
2003-08-15
期刊：
影响因子：
20.3
通讯作者：
Levy, R
中科院分区：
文献类型：
--
作者：
Akasaka, T;Lossos, IS;Levy, R
滤泡性淋巴瘤（FL）的特点是临床病程相对惰性，但该疾病经常转变为更具侵袭性的大细胞淋巴瘤，临床病程进展迅速。在本研究中，我们分析了 41 例已知随后转化为侵袭性淋巴瘤的 FL 病例，以及来自随后未转化的患者的另外 64 例 FL 样本。我们通过长距离反向聚合酶链反应（LDI-PCR）方法研究了 BCL6 基因重排。在已知发生转化的 41 例病例中，16 例 (39.0%) 在 FL 诊断时存在 BCL6 易位或缺失。在64例未知转化的病例中，9例（14.1%）检测到BCL6易位。已知发生转化的组中 BCL6 易位的发生率显着较高 (P = .0048)。在转化病例中，13例中确定了BCL6易位的伴侣，包括IGH、CIITA、U50HG、MBNL、GRHPR、LRMP、EIF4A2、RhoH/TTF和LOC92656（与NAPA类似），而在对照组中，BCL6的伴侣是IGH、CIITA、SIAT1和MBNL。 13 例中有转化前后的配对标本。在这些配对样本中，在转化后观察到BCL6易位的损失（3例）或增加（1例）。克隆性分析表明，所有这些病例都代表了原始肿瘤群体亚克隆的进化。我们的研究表明，BCL6 易位对于转化不是必需的，但 FL 中的 BCL6 易位可能构成转化为侵袭性淋巴瘤风险较高的亚组。 (C) 2003 年，美国血液学会。
Follicular lymphoma (FL) is characterized by a relatively indolent clinical course, but the disease often transforms into a more aggressive large cell lymphoma with a rapidly progressive clinical course. In the present study, we analyzed 41 cases of FL known to have subsequently transformed to aggressive lymphoma and an additional 64 FL samples from patients not subsequently transformed. We studied BCL6 gene rearrangement by the methodology of long-distance inverse polymerase chain reaction (LDI-PCR). Of the 41 cases known to transform, 16 (39.0%) harbored BCL6 translocation or deletion at the time of FL diagnosis. Among 64 cases not known to transform, BCL6 translocation was detected in 9 (14.1%). The prevalence of BCL6 translocation in the group known to transform was significantly higher (P = .0048). Among the transformation cases, the partners of the BCL6 translocation were identified in 13 cases and included IGH, CIITA, U50HG, MBNL, GRHPR, LRMP, EIF4A2, RhoH/TTF, and LOC92656 (similar to NAPA), whereas in the control group the BCL6 partners were IGH, CIITA, SIAT1, and MBNL. In 13 cases paired specimens before and after transformation available. Among these paired specimens, a loss (3 cases) or a gain (1 case) of BCL6 translocation was observed after the transformation. Analysis of clonality showed that all of these cases represented the evolution of a subclone of the original tumor population. Our study demonstrated that BCL6 translocation is not necessary for transformation but that BCL6 translocation in FL may constitute a subgroup with a higher risk to transform into aggressive lymphoma. (C) 2003 by The American Society of Hematology.