Ribonucleases of human cerebrospinal fluid: detection of altered glycosylation relative to their serum counterparts.
Ribonucleases of human cerebrospinal fluid: detection of altered glycosylation relative to their serum counterparts.
复制标题
人脑脊液核糖核酸酶：检测相对于血清对应物的糖基化改变。
DOI：
10.1021/bi00264a007
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Dekker,CA
中科院分区：
文献类型：
--
作者：
Schieven,GL;Blank,A;Dekker,CA
Gary L. Schieven、A. Blank 和 Charles A. Dekker* 摘要：通过磷酸纤维素色谱法、糖苷酶消化以及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后的活性染色，对人脑脊液 (CSF) 的核糖核酸酶 (RNase) 进行了表征。发现 CSF 含有血清中存在的主要 RNase 以及 CSF 特有的其他物种。 CSF RNase 被分为六组，分别对应于碱性血清 RNase 1-5 和血清白细胞 RNase 样酶。 CSF RNase 在凝胶上显示的分子量为 29 700 (RNase 1)、26000 (RNase 2.0)、24500 (RNase 2.1)、22900 (RNase 2.2)、19700 (RNase 3.0)、19200 (RNase 3.1)、18 700 (RNase) 3.2)、16000 (RNase 4) 和 13 900 (RNase 5)。 RNase 3.1 和 3.2 在 CSF 中发现，但在血清中未发现。最佳分辨率
Gary L. Schieven, A. Blank, and Charles A. Dekker* abstract: The ribonucleases (RNases) of human cerebro-spinal fluid (CSF) have been characterized by phosphocellulose chromatography and glycosidase digestion in conjunction with activity staining following electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. CSF was found to contain the major RNasespresent in serum plus additional species specific to CSF. The CSF RNases have been classified into six groups corresponding to the alkaline serum RNases 1-5 and the leukocyte RNase-like enzyme of serum. Molecular weights displayed by CSF RNases on gels are 29 700 (RNase 1), 26000 (RNase 2.0), 24500 (RNase 2.1), 22900 (RNase 2.2), 19700 (RNase 3.0), 19200 (RNase 3.1), 18 700 (RNase 3.2), 16000 (RNase 4), and 13 900 (RNase 5). RNases 3.1 and 3.2 are found in CSF but not in serum. Optimum resolution