Trisomy 3 may predict a poor response of gastric MALT lymphoma to Helicobacter pylori eradication therapy
Trisomy 3 may predict a poor response of gastric MALT lymphoma to Helicobacter pylori eradication therapy
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DOI：
10.3748/wjg.v11.i1.89
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发表时间：
2005-01-07
影响因子：
4.3
通讯作者：
Taniwaki, Masafumi
中科院分区：
文献类型：
--
作者：
Taji, Sawako;Nomura, Kenichi;Taniwaki, Masafumi
目的：探讨粘膜相关淋巴组织（MALT）淋巴瘤患者幽门螺杆菌根除治疗应答与肿瘤侵袭深度及染色体异常的关系，探讨非整倍体的临床价值。方法：对13例E1期胃局限性MALT淋巴瘤进行研究。根除治疗前，8例患者行内镜超声检查评估肿瘤浸润深度，其余患者行内镜检查及胃肠道系列检查。为了检测染色体异常，对诊断活检标本的石蜡包埋组织切片进行组织荧光原位杂交（FISH），对染色体3、7、12和18使用染色体特异性α -卫星DNA探针，对t（11;18）使用YAC克隆（q21;q21）。结果：13例患者中有7例对幽门螺杆菌根除治疗有完全消退（CR）。CR患者无粘膜下肿瘤侵袭。1例CR患者出现18三体，另1例CR患者同时出现12三体和18三体。所有无反应或病情进展的患者均有深部粘膜下肿瘤浸润，表现为t(11;18)（q21;q21）或3三体。在这组患者中未发现7号三体。结论：肿瘤浸润深度是E1期MALT淋巴瘤对幽门螺杆菌根除治疗反应的准确预测指标，并与染色体异常的存在密切相关。3号三体可以预测MALT淋巴瘤的侵袭性发展。(C) 2005 WJG出版社和爱思唯尔公司。版权所有。
AIM: To investigate the relation of the response to Helicobacter pylori eradication therapy to the depth of tumor invasion and chromosome abnormalities in patients with mucosa-associated lymphoid tissue (MALT) lymphoma and to determine the clinical value of aneuploidy.METHODS: We studied 13 patients with localized gastric MALT lymphoma of stage E1. Before eradication therapy, the depth of tumor invasion was assessed by endoscopic ultrasonography in 8 patients and by endoscopic examination and gastrointestinal series in the remaining patients. To detect chromosomal abnormalities, paraffin-embedded tissue sections of diagnostic biopsy specimens underwent tissue-fluorescence in situ hybridization (FISH), using chromosome-specific alpha-satellite DNA probes for chromosomes 3,7,12, and 18 and YAC clones for t(11;18)(q21;q21).RESULTS: Seven of the 13 patients had complete regression (CR) in response to H pylori eradication therapy. No patient with CR had submucosal tumor invasion. Trisomy 18 was seen in 1 patient with CR, and both trisomies 12 and 18 were present in another patient with CR. All patients with no response or progressive disease had deep submucosal tumor invasion and showed t(11;18)(q21;q21) or trisomy 3. Trisomy 7 was not detected in this series of patients.CONCLUSION: The depth of tumor invasion is an accurate predictor of the response of stage E1 MALT lymphoma to H pylori eradication therapy and is closely associated with the presence of chromosomal abnormalities. Trisomy 3 may predict the aggressive development of MALT lymphoma. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.