Swept source optical coherence microscopy using a 1310 nm VCSEL light source
Swept source optical coherence microscopy using a 1310 nm VCSEL light source
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DOI：
10.1364/oe.21.018021
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发表时间：
2013-07-29
期刊：
影响因子：
3.8
通讯作者：
Fujimoto, James G.
中科院分区：
文献类型：
--
作者：
Ahsen, Osman O.;Tao, Yuankai K.;Fujimoto, James G.
我们展示了高速，扫频光源光学相干显微镜（OCM）使用MEMS可调谐垂直腔面发射激光器（VCSEL）光源。光源具有280 kHz的扫描速率，提供560 kHz的双向轴向扫描速率。扫描带宽为117 nm，中心为1310 nm，对应于空气中13.1 μ m的轴向分辨率，对应于组织中8.1 μ m（9.6 μ m光谱形状）。不同物镜的色散失配得到了数值补偿，使得放大率和视场可以很容易地改变。使用可互换的40倍、20倍和10倍物镜，分别以类似于600 μ m × 600 μ m、类似于1 mm × 1 mm和类似于2 mm × 2 mm的视场（FOV），以0.86 μ m - 3.42 μ m的横向分辨率采集OCM图像。通过数值计算，修正了光束扫描时光程长度的寄生变化。这些功能使扫频源OCM能够与各种现有的扫描显微镜集成。通过连续获取相邻重叠的显微镜场并在后处理中将它们组合来生成大FOV马赛克。对新鲜的人结肠、甲状腺和肾脏标本进行离体成像，并与匹配的组织学切片进行比较，证明OCM能够对组织标本进行成像。(C)2013年美国光学学会
We demonstrate high speed, swept source optical coherence microscopy (OCM) using a MEMS tunable vertical cavity surface-emitting laser (VCSEL) light source. The light source had a sweep rate of 280 kHz, providing a bidirectional axial scan rate of 560 kHz. The sweep bandwidth was 117 nm centered at 1310 nm, corresponding to an axial resolution of 13.1 mu m in air, corresponding to 8.1 mu m (9.6 mu m spectrally shaped) in tissue. Dispersion mismatch from different objectives was compensated numerically, enabling magnification and field of view to be easily changed. OCM images were acquired with transverse resolutions between 0.86 mu m - 3.42 mu m using interchangeable 40X, 20X and 10X objectives with similar to 600 mu m x 600 mu m, similar to 1 mm x 1 mm and similar to 2 mm x 2 mm field-of-view (FOV), respectively. Parasitic variations in path length with beam scanning were corrected numerically. These features enable swept source OCM to be integrated with a wide range of existing scanning microscopes. Large FOV mosaics were generated by serially acquiring adjacent overlapping microscopic fields and combining them in post-processing. Fresh human colon, thyroid and kidney specimens were imaged ex vivo and compared to matching histology sections, demonstrating the ability of OCM to image tissue specimens. (C) 2013 Optical Society of America