The human LGALS3 (galectin-3) gene:: Determination of the gene structure and functional characterization of the promoter
The human LGALS3 (galectin-3) gene:: Determination of the gene structure and functional characterization of the promoter
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DOI：
10.1006/abbi.1997.0447
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发表时间：
1998-01-01
影响因子：
3.9
通讯作者：
Wang, JL
中科院分区：
文献类型：
--
作者：
Kadrofske, MM;Openo, KP;Wang, JL
Galectin-3 是一种 β-半乳糖苷特异性凝集素，是一种前 mRNA 剪接因子。在这里，我们报告了人类 LGALS3 (galectin-3) 基因的基因组结构和启动子的功能特征。基因组 DNA 的 Southern 印迹分析显示，galectin-3 由人类基因组中的单个基因编码，该基因由 6 个外显子和 5 个内含子组成，总共跨越约 17 kilobase (kb)。基于引物延伸和核糖核酸酶保护分析，有两个转录起始位点位于外显子 I-内含子 1 边界上游 52 和 50 个核苷酸 (nt) 处，此处定义为 + 1a和+1b，分别。翻译起始位点位于外显子 II 中，核糖核蛋白样 N 末端结构域包含脯氨酸-甘氨酸-丙氨酸-酪氨酸 (PGAY) 重复基序，完全位于外显子 III 内。碳水化合物识别序列完全位于外显子 V 内。包含 -836 至 +141 nt（相对于 +1a）的基因组片段在连接到荧光素酶报告基因并瞬时转染到 HeLa 细胞或人二倍体成纤维细胞中时具有显着的启动子活性，静止成纤维细胞具有较低的启动子活性，但在添加血清后活性增加 100 倍，启动子中的血清响应激活区域位于 -513 和 -339 nt 之间-339 至 -229 nt 之间；额外的激活区域可能位于-105和-15 nt之间。由于galectin-3是一种立即早期基因，其表达依赖于细胞的增殖状态，因此本研究为确定肿瘤或细胞衰老中转录调控的分子机制提供了基础。 (C) 1998 年学术出版社。
Galectin-3 is a beta-galactoside-specific lectin that is a pre-mRNA splicing factor. Here we report the genomic organization of the human LGALS3 (galectin-3) gene and functional characterization of the promoter. Southern blot analysis of genomic DNA revealed that galectin-3 is coded by a single gene in the human genome, The gene is composed of six exons and five introns, spanning a total of similar to 17 kilobases (kb), Based on primer extension and ribonuclease protection analyses, there are two transcription initiation sites located 52 and 50 nucleotides (nt) upstream of the exon I-intron 1 border, and defined here as + 1a and + 1b, respectively. The translation start site is in exon II, The ribonucleoprotein like N-terminal domain, containing the proline-glycine-alanine-tyrosine (PGAY) repeat motif, is found entirely within exon III. The carbohydrate recognition sequence is found entirely within exon V. Genomic fragments encompassing -836 to +141 nt (relative to +1a) have significant promoter activity when linked to the luciferase reporter gene and transiently transfected into HeLa cells or human diploid fibroblasts, Quiescent fibroblasts have low promoter activity but the activity increases 100-fold following serum addition, Serum responsive activation regions in the promoter are located between -513 and -339 nt and between -339 and -229 nt; an additional activation region may be located between -105 and -15 nt, Because galectin-3 is an immediate-early gene whose expression is dependent on the proliferative state of the cell, this study provides the basis for determining the molecular mechanisms of transcriptional regulation in neoplasia or cellular senescence. (C) 1998 Academic Press.