Nonmyeloablative Bone Marrow Transplantation of BXSB Lupus Mice Using Fully Matched Allogeneic Donor Cells from Green Fluorescent Protein Transgenic Mice1
Nonmyeloablative Bone Marrow Transplantation of BXSB Lupus Mice Using Fully Matched Allogeneic Donor Cells from Green Fluorescent Protein Transgenic Mice1
复制标题
使用来自绿色荧光蛋白转基因小鼠的完全匹配的同种异体供体细胞对 BXSB 狼疮小鼠进行非清髓性骨髓移植1
DOI：
10.4049/jimmunol.172.9.5415
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
R. Good
中科院分区：
文献类型：
--
作者：
O. Jones;A. Steele;Joe M. Jones;Y. Marikar;Yenhui Chang;A. Feliz;R. Cahill;R. Good
雄性BXSB小鼠，一种系统性红斑狼疮的小鼠模型，在20周龄时使用MHC匹配的供体细胞和非清髓性预处理（550 cGy照射）进行骨髓移植（BMT）。移植小鼠和照射对照组进行了为期20周的跟踪。将绿色荧光蛋白转基因小鼠用作供体，以允许追踪供体细胞和测定嵌合性。与未治疗的小鼠相比，放射对照组在移植后10周时肾脏病理学有所减轻，但在20周时却没有减轻，而非清髓性BMT小鼠在两个时间间隔内的病理学均显着减轻。BMT也降低了老年BXSB小鼠的单核细胞增多症特征，但治疗并不能阻止抗dsDNA抗体的产生。在脾脏和骨髓中，24-40%的供体CD 45阳性细胞达到稳定的嵌合体，并且没有临床移植物抗宿主病的证据。在大多数受体器官中检测到供体细胞，特别是胸腺和肾小球。结果表明，完全耗尽成熟淋巴细胞或祖细胞干细胞是不需要控制狼疮肾炎BXSB小鼠。
Male BXSB mice, a mouse model of systemic lupus erythematosus, were given bone marrow transplants (BMT) at 20 wk of age using MHC-matched donor cells and nonmyeloablative conditioning (550 cGy irradiation). Transplanted mice and irradiation controls were followed for a period of 20 wk. Mice transgenic for green fluorescent protein were used as donors to allow tracking of donor cells and a determination of chimerism. Radiation controls had reduced renal pathology at 10 wk posttransplant, but not at 20 wk compared with untreated mice, while nonmyeloablative BMT mice had significantly reduced pathology at both time intervals. The monocytosis characteristic of older BXSB mice was also reduced by BMT, but the treatment did not prevent production of Ab to dsDNA. A stable chimerism of 24–40% donor CD45-positive cells was achieved in spleen and bone marrow, and there was no evidence of clinical graft vs host disease. Donor cells were detected in most recipient organs, notably the thymus and renal glomeruli. The results suggest that complete depletion of mature lymphocytes or of progenitor stem cells is not required to control lupus nephritis in BXSB mice.
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影响因子：
6.2
作者：
Manilay, JO;Pearson, DA;Sykes, M
通讯作者：
Sykes, M
影响因子：
6.2
作者：
Nikolic, B;Lei, H;Sykes, M
通讯作者：
Sykes, M
DOI：
10.1073/pnas.87.21.8341
发表时间：
1990-11-01
影响因子：
11.1
作者：
IKEHARA, S;KAWAMURA, M;GOOD, RA
通讯作者：
GOOD, RA
DOI：
10.1073/pnas.82.8.2483
发表时间：
1985-01-01
影响因子：
11.1
作者：
IKEHARA, S;GOOD, RA;HAMASHIMA, Y
通讯作者：
HAMASHIMA, Y