CCR4 and CAF1 deadenylases have an intrinsic activity to remove the post-poly(A) sequence.
CCR4 and CAF1 deadenylases have an intrinsic activity to remove the post-poly(A) sequence.
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DOI：
10.1261/rna.057679.116
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发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
Tomari Y
中科院分区：
文献类型：
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作者：
Niinuma S;Fukaya T;Tomari Y
MicroRNAs(MiRNAs)招募CCR4-Not复合体，其中包含两个死烯基酶CCR4和CAF1，以促进聚(A)尾巴的缩短。虽然CCR4和CAF1普遍对聚(A)RNA底物有强烈的偏好，但从酵母到人类的研究表明，它们在体外也可以不同程度地去除非A残基。然而，目前尚不清楚CCR4和CAF1是如何去除非A序列的。在这里，我们证明了果蝇miRNAs可以促进3‘-末端非A残基的去除，但前提是上游存在Poly(A)序列。这种非A去除反应由CCR4和CAF1直接催化，并依赖于内部聚(A)序列和下游非A序列的长度之间的平衡。这些结果表明，CCR4-NOT复合体具有内在的活性，可以去除聚(A)尾下游的3‘-末端非A修饰。
MicroRNAs (miRNAs) recruit the CCR4–NOT complex, which contains two deadenylases, CCR4 and CAF1, to promote shortening of the poly(A) tail. Although both CCR4 and CAF1 generally have a strong preference for poly(A) RNA substrates, it has been reported from yeast to humans that they can also remove non-A residues in vitro to various degrees. However, it remains unknown how CCR4 and CAF1 remove non-A sequences. Herein we show that Drosophila miRNAs can promote the removal of 3′-terminal non-A residues in an exonucleolytic manner, but only if an upstream poly(A) sequence exists. This non-A removing reaction is directly catalyzed by both CCR4 and CAF1 and depends on the balance between the length of the internal poly(A) sequence and that of the downstream non-A sequence. These results suggest that the CCR4–NOT complex has an intrinsic activity to remove the 3′-terminal non-A modifications downstream from the poly(A) tail.
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