A NOVEL VIRAL ONCOGENE WITH STRUCTURAL SIMILARITY TO PHOSPHOLIPASE-C
A NOVEL VIRAL ONCOGENE WITH STRUCTURAL SIMILARITY TO PHOSPHOLIPASE-C
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DOI：
10.1038/332272a0
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发表时间：
1988-03-17
期刊：
影响因子：
64.8
通讯作者：
HANAFUSA, H
中科院分区：
文献类型：
--
作者：
MAYER, BJ;HAMAGUCHI, M;HANAFUSA, H
从急剧转化的逆转录病毒中分离出了大量的癌基因。到目前为止，这些病毒癌基因的产物有蛋白激酶、核蛋白、生长因子或GTP结合蛋白1、2。我们克隆了以前未鉴定的禽肉瘤病毒CT10，并对其基因组进行了测序。该病毒编码一种名为p47gag-Crk的蛋白质，其序列与非受体类酪氨酸激酶的氨基末端非催化区有许多相似之处。此外，p47gag-crk的结构与牛脑磷脂酶C的180个氨基酸区域有惊人的相似性。生化数据表明，p47gag-crk可以激活一个或几个内源性酪氨酸激酶。
Numerous oncogenes have been isolated from acutely transforming retroviruses. To date, the products of these viral oncogenes have been protein kinases, nuclear proteins, growth factors, or GTP-binding proteins1,2. We have cloned the previously uncharacterized avian sarcoma virus CT10 and sequenced its genome. This virus encodes a protein, p47gag-crk, that has blocks of sequence similarity to the amino-terminal, non-catalytic region of the non-receptor class of tyrosine kinases. In addition, the structure of p47gag-crkhas striking similarity to a 180-amino acid region of bovine brain phospholipase C. Biochemical data suggest that p47gag-crkactivates one or several endogenous tyrosine kinases.