Effects of reactive oxygen species from activated leucocytes on human sperm motility, viability and morphology
Effects of reactive oxygen species from activated leucocytes on human sperm motility, viability and morphology
复制标题
活性白细胞活性氧对人类精子活力、活力和形态的影响
DOI：
10.1111/j.1439-0272.2011.01252.x
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发表时间：
2012-05-01
期刊：
影响因子：
2.4
通讯作者：
Shi, H.
中科院分区：
文献类型：
--
作者：
Shi, T. -Y.;Chen, G.;Shi, H.
累积的数据表明，炎症可以增加活性氧（ROS）的水平，这有助于精子功能受损和男性不育。因此，我们认为炎症介导的男性和女性生殖道中ROS的产生阻碍了精子受精。为了验证这一假设，应用佛波醇肉豆蔻酸酯（PMA）与多形核白细胞（PMNs）来产生内源性活性氧。我们评估了ROS对人精子运动力、存活力和线粒体膜电位（MMP）的时间依赖性影响。结果表明，PMA和PMNs处理后，人精子的活动力显着下降到50%，在第1天和第4天的15%，分别与阴性对照组相比（P = 0.012）。PMA + PMNs处理后第4天精子活力下降（P = 0.028）。PMA + PMN组精子MMP在第2 ~ 4天较对照组显著降低（P = 0.019）。总之，4天培养方法提供了对精子质量的准确评估，尤其是精子活力和MMP。我们的研究结果表明，内源性炎症增加ROS水平，这可能会导致精子氧化损伤。此外，精子活力可能是这种损伤的最早和最敏感的指标之一。
The accumulated data suggest that inflammation can increase the level of reactive oxygen species (ROS), which contribute to impaired sperm function and male infertility. Therefore, we propose that inflammation-mediated production of ROS in male and female reproductive tracts hinder sperm fertilisation. To test this hypothesis, phorbol myristate acetate (PMA) with polymorphonuclear leucocytes (PMNs) was applied to generate endogenous ROS. We evaluated the time-dependent effects of ROS on human sperm motility, viability and mitochondrial membrane potential (MMP). The results showed that after treatment with PMA and PMNs, the motility of human spermatozoa significantly decreased to 50% on Day 1 and 15% on Day 4 compared with that of the, respectively, negative controls (P = 0.012). The viability of human spermatozoa decreased on Day 4 of PMA + PMNs treatment (P = 0.028). The MMP of human spermatozoa significantly decreased from Day 2 to Day 4 in the PMA + PMN group compared with that of the controls (P = 0.019). Taken together, the 4-day cultivation approach provided an accurate evaluation of sperm quality, especially sperm motility and MMP. Our findings indicated that endogenous inflammation increased ROS levels, which might induce sperm oxidative damage. Additionally, sperm motility might be one of the earliest and most sensitive indicators of this damage.