Angiotensin II immunoreactivity coexists with renin in the juxtaglomerular granular cells of the kidney.
Angiotensin II immunoreactivity coexists with renin in the juxtaglomerular granular cells of the kidney.
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血管紧张素 II 免疫反应性与肾素共存于肾脏的肾小球旁颗粒细胞中。
DOI：
10.1073/pnas.78.6.3897
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发表时间：
1981
影响因子：
11.1
通讯作者：
Inagami,T
中科院分区：
文献类型：
--
作者：
Celio,MR;Inagami,T
血管紧张素II(AII)的多种生理功能通常被认为是由血液循环中产生的多肽介导的。除了AII形成的这种细胞外机制外，我们还获得了肾脏细胞内合成AII的免疫组织化学证据。大鼠被灌流固定剂，肾脏的石蜡切片用抗肾素(EC 3.4.99.19)、AII和肾素-血管紧张素系统的其他成分的抗血清处理。肾素免疫反应常见于肾小球传入血管中的上皮样颗粒细胞。所有免疫反应均存在于同一细胞内。此观察指向肾小球旁上皮样颗粒细胞内AII的产生。然后在间质液体和血液中与肾素一起释放AII。AII的旁分泌可对肾小球血管张力产生局部调节作用。
The multiple physiologic functions of angiotensin II(AII) are generally supposed to be mediated by the peptide generated in the blood circulation. In addition to this extracellular mechanism of AII formation, we have obtained immunohistochemical evidence for the intracellular synthesis of AII in the kidney. Rats were perfused with fixative, and paraffin sections of the kidneys were processed with antisera against renin (EC 3.4.99.19), AII, and other components of the renin--angiotensin system. Renin immunoreactivity was regularly observed in the epithelioid granular cells in the media of the afferent vessel of the glomerulus. AII immunoreactivity was found to coexist within the same cells. This observation points to an intracellular production of AII in the juxtaglomerular epitheloid granular cells. AII may then be released concomitantly with renin in the interstitial fluid and in the blood. The paracrine secretion of AII could exert a local regulatory influence on the tonus of the glomerular vessels.