Duplicate FLORICAULA/LEAFY homologs zfl1 and zfl2 control inflorescence architecture and flower patterning in maize
Duplicate FLORICAULA/LEAFY homologs zfl1 and zfl2 control inflorescence architecture and flower patterning in maize
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DOI：
10.1242/dev.00457
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发表时间：
2003-06-01
期刊：
影响因子：
4.6
通讯作者：
Doebley, J
中科院分区：
文献类型：
--
作者：
Bomblies, K;Wang, RL;Doebley, J
金鱼草属植物的同源转录因子FLORICAULA和拟南芥属植物的LEAFY在花分生组织身份和花图案中具有保守的作用。虽然FLORICANOL/LEAFY同源基因在花发育中的作用已在许多双子叶植物中得到证实，但对这些分生组织身份基因在更远的有花植物单子叶植物中的功能知之甚少。我们利用反向遗传学研究了两个重复的FLORICARBON/LEAFY同源基因在玉米（Zea mays L.）SSP. Mays）-具有与双子叶植物物种显著不同的花和花序形态的单子叶植物物种。转座子插入到玉米基因，zfl 1和zfl 2，导致花器官的身份和图案的破坏，以及在花序结构和营养生殖阶段的过渡缺陷。我们的研究结果表明，这些基因与双子叶植物的花和花序图案的保守角色。zfl 1的表型; zfl 2双突变体表明，这些玉米FLORICARBON/LEAFY同源物作为ABC花器官身份基因的上游调节因子，这沿着先前发表的工作，表明调控花发育的转录网络在单子叶植物和双子叶植物之间至少部分保守。我们的数据还表明，zfl基因可能在控制玉米花序叶序的数量方面发挥了新的作用，与它们作为控制玉米及其祖先大刍草花序结构差异的数量性状基因座的候选资格一致。
The homologous transcription factors FLORICAULA of Antirrhinum and LEAFY of Arabidopsis share conserved roles in flower meristem identity and floral patterning. While roles for FLORICAULA/LEAFY homologs in flower development have been demonstrated in numerous dicots, little is known about the function of these meristem identity genes in the more distantly related flowering plants, the monocots. We used reverse genetics to investigate the role of two duplicate FLORICAULA/LEAFY homologs in maize (Zea mays L. ssp. mays) - a monocot species with dramatically different flower and inflorescence morphology from that of dicot species. Transposon insertions into the maize genes, zfl1 and zfl2, led to a disruption of floral organ identity and patterning, as well as to defects in inflorescence architecture and in the vegetative to reproductive phase transition. Our results demonstrate that these genes share conserved roles with their dicot counterparts in flower and inflorescence patterning. The phenotype of zfl1; zfl2 double mutants suggests that these maize FLORICAULA/LEAFY homologs act as upstream regulators of the ABC floral organ identity genes, and this along with previously published work, indicates that the transcriptional network regulating flower development is at least partially conserved between monocots and dicots. Our data also suggest that the zfl genes may play a novel role in controlling quantitative aspects of inflorescence phyllotaxy in maize, consistent with their candidacy for quantitative trait loci that control differences in inflorescence structure between maize and its progenitor, teosinte.