Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice
Overexpression of OsCOIN, a putative cold inducible zinc finger protein, increased tolerance to chilling, salt and drought, and enhanced proline level in rice
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DOI：
10.1007/s00425-007-0548-5
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发表时间：
2007-09-01
期刊：
影响因子：
4.3
通讯作者：
Chong, Kang
中科院分区：
文献类型：
--
作者：
Liu, Kaimao;Wang, Lei;Chong, Kang
水稻（Oryza sativa L.）植物对低温敏感，特别是在幼苗发育的早期阶段。在此，分离并表征了一种新的冷诱导基因，命名为OsCOIN（（O）在bar ryza下（s）在bar ativa下（c）在bar old下-（i）在bar inducible下）。结果表明，OsCOIN蛋白是一种RING指蛋白，定位于细胞核膜和细胞质膜。OsCOIN在水稻各器官中均有表达，并受低温、阿坝、盐和干旱的强烈诱导。过量表达OsCOIN基因的转基因水稻对低温、盐胁迫和干旱胁迫的耐受性显著增强，同时OsP 5CS表达上调，细胞内脯氨酸含量增加。
Rice (Oryza sativa L.) plant is sensitive to chilling, particularly at early stages of seedling development. Here a novel cold-inducible gene, designated OsCOIN ((O) under bar ryza (s) under bar ativa (c) under bar old-(i) under bar nducible), was isolated and characterized. Results showed that OsCOIN protein, a RING finger protein, was localized in both nuclear and cytoplasm membrane. OsCOIN is expressed in all rice organs and strongly induced by low temperature, ABA, salt and drought. Overexpression of OsCOIN in transgenic rice lines significantly enhanced their tolerance to cold, salt and drought, accompanied by an up-regulation of OsP5CS expression and an increase of cellular proline level.