Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants.
Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants.
复制标题
噬菌斑大小表型作为生成痘苗病毒重组体的选择标记。
DOI：
10.1128/jvi.63.2.997-1001.1989
复制
发表时间：
1989
影响因子：
5.4
通讯作者：
Esteban,M
中科院分区：
文献类型：
--
作者：
Rodriguez,JF;Esteban,M
本文提供了一种新的筛选表达外源基因的重组痘苗病毒的方法。该方法基于编码病毒14，000分子量包膜蛋白的基因的使用，该病毒包膜蛋白将牛痘病毒变体的小噬斑大小表型拯救为大噬斑大小病毒。在目视检查大病毒噬斑后，容易获得重组体的选择。
In this report, we provide a new method for selection of vaccinia virus recombinants expressing foreign genes. The method is based on the use of the gene encoding the viral 14,000-molecular-weight envelope protein that rescues the small-plaque-size phenotype of a vaccinia virus variant to large-plaque-size virus. Selection of recombinants is easily obtained after visual inspection of large viral plaques.